棘椒幼苗低温胁迫的生理响应与转录组分析

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中图分类号：S641.3 文献标志码：A 文章编号：1673-2871（2026）01-012-14

Abstract：PepperisoneofthemostimportantvegetablecropsinChina，withacultivationscaleandeconomicvaluerankingamongthetopinthe world.However，lowtemperature significantly inhibits the growthofpepper.This study focuses onthecoldtolerantmaterialKC13andthecoldsensitive materialKC14.Throughphenotypeidentification，celllarphysiology，biochemical indicators，andtranscriptomeanalysis，underlying the diferences incold tolerance betweenthe two materialswereexplored.Theresultsshowedthatlowtemperature stresscaused wiltingofleavesanddamage tocelstructure nthecoldsensitive material KC14，whereas there was no significantchange inthecoldtolerant material KCl3.Biochemical analysis sows that the totalchlorophyllcontent ofcoldresistant materials islessaffectedbylowtemperatures， and has higher POD activity and soluble sugar content，slower MDA accumulation rate，compared to KC14. Transcriptome sequencing data KEGG enrichment analysis and RT-qPCR showed that the expression paterns of key genes involvedin the oxidative phosphorylation pathway were specificallyup-regulated in KC13.In summary，cold tolerant peppermaterialKC13enhancesplant stress resistancebymaintaining photosynthetic stabilityenhancingantioxidantcapacity，accumulatingosmoregulatorysubstancesandactivatingoxidativephosphorylationpathway.Theresearchresultslaid the foundation for molecular breeding of cold tolerance in pepper.

Key words： Pepper; Low temperature stress; Transcriptome analysis; Differentially expressed gene; Oxidative phosphory lation

辣椒（CapsicumannuumL.）是我国重要的经济蔬菜作物，种植规模与产业价值位居全球之首。（剩余15194字）