糙皮侧耳Hsp基因家族鉴定及其在热胁迫下的表达分析

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中图分类号： S646.l+4l 文献标志码：A 文章编号：1673-2871（2026）01-026-13

Abstract： Heat shock protein（Hsp）is aclass of molecularchaperones produced byorganismsunder heat stress orother environmentalstimuliandplaysimportantroles inabioticstressresponses.Inthisstudy，theHspgene familyinthePleurotus ostreatus CCMSSCCoo389 strain was identified by bioinformatics，and itsexpresson patterns under heatstress were analyzed.The results showed that a total of 27 PoHsps were identified in the P. ostreatus genome and were unevenly distributed across 11 chromosomes.The analysis resultsofcis-acting elements in gene promoters show that hormone-responsivecis-acting elementsacountforthelargestproportion.Furtherinvestigationrevealedthatunderheatstresatdifferenttemperatures，theexpressonpatternsofPoHspscouldbebroadlyclassified intofivecategories：PoHspl9expression gradually decreased with increasing temperature; PoHsp7and PoHsp24 expression did not significantly change underdifferenthatstresconditions;ndtheexpressiopatersofp，PoHp4，PoHp，andPHspl5prestedsimilarexpressionpatterns，allofwhichinitiallydecreasedbutthenincreased;however，theotherPoHspsgraduallyceased with increasingstress temperature.These findingssuggest thatPoHspsmay have functional diferences inresponse to various stressconditions.Thisstudy providesafoundationforfurtherfunctionalcharacterizationoftheHspgenefamilyin P. （20 ostreatusunderabioticstress.

Keywords：Pleurotus ostreatus;Hsp gene family;Heat stress;Bioinformatics analysis

热激蛋白（heat shock protein，Hsp）在生物体中广泛存在，是生物体在热胁迫或其他环境刺激下产生的一类分子伴侣蛋白，能够参与蛋白质的折叠、转运、降解以及蛋白复合体的组装等过程-2]。（剩余13319字）