镰刀菌属植物病原真菌CFEM蛋白的理化性质及特征分析

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中图分类号 S432.4 文献标识码A文章编号 0517-6611（2025）18-0121-04doi：10.3969/j.issn.0517-6611.2025.18.026

Analysis of Physicochemical Propertiesand Characteristicsof CFEMProteins inPlant Pathogenic Fungiof theFusariumGenus ZHUYou-peng'，SHENYan，CHENMin1*（1.BijieForestryBureau，Bijie，Guizhou517O;2.Dafang CoutyAgricultureandRuralBu reau，Bijie，Guizhou 551700）

AbstractTosstematicalleluciatetehysicoemicalcaracteristisofEMproteinsinplant-athogenicfungiofthgensFusm， focusedon6typicalFusaimspecs toonuctsequeesreeningdharacteriticalysisofEprotes.Intiall，CanddateC proteiequencefromthsesixFusarimspeciswereobtaedbysearchingtePfamdatabaseusingthkeyword“CFEM".Subsequentlyte presencef a“CFEM”domain wasverifiedusingSMART.TheobtainedCFEMproteinsequences werethensystematicallanalyzedusingvariousnlinebEselet（lid hydrophilicityellaocatoducueooaulsicadatotsi cies are predominantly acidic（ pI<6.01 ），stable（instabilityindex <40.01 ），andhydrophobic（hydropathicity >0 ）.Subcellular localization predictionsevealedtatmostproteisareprimarlylcatedinthplasmamembanitonlyinoritargetigsecificrganlesuchs mitochondria.Secondary structure analysis showed that α -helices and random coils constitute higher proportion，while β -sheets account for less than 4% .All identified proteins contain the conserved CFEM domain and can be clasified into two types：pure CFEMand CFEM + TM （ transmembranedmain）.ThCFEdomainithaconservedainoacidlengthrangingbtween6O-66aontais6spacedhighlyoseedcs teine residues，demonstrating a high degree of conservation.

KeyWordsFusarium;CFEM protein;Physicochemical properties;Bioinformatics analysis

CFEM（commonin fungal extracellularmembrane）蛋白是真菌中特有的一类蛋白，其核心标志是含1个或多个CFEM保守结构域，该结构域通常由约60个氨基酸组成，且含有8个间隔排列的半胱氨酸，在真菌生长发育调控和与宿主互作的致病过程中具有重要作用[2-3]。（剩余6976字）