基于正交设计的杉木丛生芽增殖关键因素解析与优化

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中图分类号 S791.27 文献标识码A 文章编号 1007-7731（2025）20-0074-04

DOI号10.16377/j.cnki.issn1007-7731.2025.20.017

AbstractTo screen the medium formula suitable for theproliferationof clump-forming buds，this studyused the ‘Yang-061’clonal Cunninghamia lanceolataas the experimental material.The efects of 6-BA （0.5，1.0，1.5mg/L）， NAA （0.10， 0.15， 0.20mg/L ），and riboflavin （O， O.1， O.2 g/L） on the proliferation coefficient of clump-forming buds of Cunninghamia lanceolata were investigated by using a 3-factor 3-level L9 （ 33 ）orthogonal design experiment. The results indicated that the optimal medium formula for clump-forming bud proliferation was MS medium+0.5 mg/L 6-BA+0.2 mg/L NAA+0.2 g/L riboflavin+40 g/L sucrose+6 g/L agar， with a pH of 5.8.Under this condition，the proliferationcoeficientof theclump-formingbuds wasthehighest，whichwas3.84.Theprimaryandsecondaryorderof the effects of various factorson the proliferationof clump-forming buds of Cunninghamia lanceolata was 6-BA> riboflavin > NAA.The change in 6-BA concentration was the main factor afecting the proliferation coefficient of clumpformingbudsofCunninghamia lanceolata，andtheinteractionamong 6-BA，NAAandriboflavin hadarelativelysmall effcton theproliferation of clump-forming budsof Cunninghamia lanceolata.Thisarticle providesareference for large-scale seedling cultivation of Cunninghamia lanceolata.

Keywords Cunninghamia lanceolata; proliferation of clump-forming buds; orthogonal design; 6-BA

杉木（Cunninghamialanceolata）作为南方地区重要的速生用材树种之一，其木材纹理通直、材质轻软且富有弹性，广泛应用于建筑、家具制造等多个领域，在林业经济中占据着重要地位[1-3]。（剩余5358字）