外源茉莉酸甲酯对猴樟幼苗耐碱性的影响

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中图分类号 S792.23 文献标识码A 文章编号 1007-7731（2025）20-0069-05

DOI号 10.16377/j.cnki.issn1007-7731.2025.20.016

AbstractTo analyze the effect of exogenous methyl jasmonate （MeJA）on the alkali resistance of Camphora bodinieriseedlings，inthisstudy，Camphora bodinieri seedlingswereusedasmaterialsand treatedwith exogenous MeJAat concentrations of O （control），20o，500，and 1000μmol/L ，respectively. The content of photosynthetic pigments，the degree of lipid peroxidation incell membranes，theactivityof antioxidant enzymes and the expression levels of keygenes for flavonoid metabolism in theseedlings of Camphora bodinieri under alkali stressunder each treatment weredetermined，and thealkali resistanceof theseedlingswasanalyzedbythe membership function method.The results indicated that treatment with 2OO and 500 μmol/L exogenous MeJA could increase the content of photosynthetic pigments and soluble sugar of Camphora bodinieri seedlings，reduce the content of MDA and relative electricalconductivityenhance theactivityofantioxidantenzymes，andactivate the expressionlevelsof genesrelated toflavonoid metabolism.Among them，thecontentsof chlorophylla，chlorophyllbandcarotenoids inthe leavesof seedlings treated with 200μmol/L MeJA increased by 7.13% ， 57.31% and 21.93% respectively compared with the control. The MDA content in the leaves of seedlings decreased by 34.41% compared with the control，and the relative conductivitydereasedbyl2.66 percentage points compared tothecontrol，thecontentof soluble sugar increased by 44.78% compared with the control， while the CAT activity and POD activity in the leaves increased by 17.71% and 65.49% respectively compared with the control. The expresson levels of CbCHS，CbDFR and CbUFGT genes increased by 248.88% ， 158.71% and 151.07% respectively compared with the control group.The comprehensive evaluationresults showed that exogenous MeJA treatmentcould enhance the alkali resistance of Camphora bodinieri seedlings.Among them，the treatment with 200 p mol/LMeJAhadthebesteffect.Itwasspeculated thatMeJA improves thealkaliresistanceof plantsbyactivating theflavonoidmetabolicpathway.Thisarticle providesa reference for studying the molecular mechanism by which MeJA enhances the alkali resistance of Camphora bodinieri.

KeywordsCamphora bodinieri; alkali resistance;methyl jasmonate;flavonoid metabolism;antioxidant enzyme activity

猴樟（Camphorabodinieri）为樟科樟属常绿树种，是重要的园林绿化和油料加工树种之一，也是制造食品添加剂、香料、化妆品、抗菌试剂等的重要材料。（剩余7003字）