烟草赤星病土壤拮抗放线菌的筛选

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中图分类号 S435.72 文献标识码A 文章编号 1007-7731（2025）18-0087-04

DOI号 10.16377/j.cnki.issn1007-7731.2025.18.022

Screeningof soil antagonistic actinomycetesagainst tobacco brownspot

LU Zhengzheng1，2 WANG Fei³LI Xiaoxia1XIAO Zhongjiu1YANG Xuyan1，2

（1Zunyi Normal University， Zunyi 563002， China; （2 2 Guizhou Province to Create A Keen Sense of Identity of the Chinese Nation，Zunyi Normal University Research Base， Zunyi 563002， China; 3Yunnan Agricultural University，Kunming 650oo， China）

AbstractTo screen foractinomycetes with antagonistic efects against tobacco brown spot disease，the experiment began with microscopic morphological observation of the tobacco brown spot.Actinomycetes were isolated from the rhizospheresoilof healthy tobacco plantsusing the dilution plateseparationmethod.Meanwhile，an in vitro plate confrontationassay wasconducted toanalyze theirantagonistic efects.Theresultsshowed that the conidia of the tobacco brown spot pathogen were light brown and club-shaped，while the hyphae were colorless and transparent.A totalof 59actinomycete strains were isolatedfrom thesoil，exhibiting diversecolonycolorsand tightlyintertwined hyphae.The plate-confrontationassayresults indicated that7actinomycete strains exhibited inhibitory efects against tobacco brownspot disease.Theaverage inhibition zone diameters over5consecutive days were as follows：YF-33 （20.46 mm） >YF-49 （15.12 mm） > YF-5 （14.60 mm） > YF-1 （11.76mm）>YF-48（11.22mm）>YF-41（9.64mm）> YF-55 （9.44 mm）.Among them，YF-33，YF-49，andYF-5 showedacontinuous increasing trend ininhibition zone diameters overthe5days，with thediameters reaching 23.13mm，16.33 mm，and17.33mm，respectively，onthe 7th day.OveralltheactinomycetesYF-33，YF-49，andYF-5demonstrated effctive，stable，andprolongedantagonistic effcts，providing valuableinsightsfor the biological control of tobaccobrownspot diseaseusing antagonistic microorganisms.

Keywordstobacco brown spot; rhizosphere soil; actinomycetes;antagonism; biological control

烤烟作为重要的经济作物之一，对稳定烟农收人、促进农业产业结构优化等具有重要意义。（剩余4576字）