马铃薯茎尖脱毒组织培养技术
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中图分类号S532 文献标识码A 文章编号 1007-7731（2025）18-0034-04
DOI号 10.16377/j.cnki.issn1007-7731.2025.18.009
Potato stem tip virus-free tissue culture technique
LI Yajun1SHI Guangcheng1LILi1LI Bo1 GAO Panpan1 FAN Fenfen1 ZHANG Xueting1 HU Nana² （1Zichang Potato Industry Development Center， Zichang 717300， China; ²Zichang Potato Research Institute， Zichang 71730o， China）
AbstractThe virus-free tisseculture technology using potato stem tips is able to restore varietycharacteristics and improve potato yieldandquality，making itone ofthekeybiotechnologies inseed potato production.Theentire procesof virus-free tissue culture technologyusing potato stem tips was systematically elaborated，and its common problems along withcontrolstrategies were summarized.Thetechnical procedure involved pretreatmentof explants，shoot tipdissection， primarycultureof plantlets，virusdetection，andinvitropropagationof plantlets.During theprimaryculturestage，the explantswere sterilizedand pretreated，and thencultured inaspecificmedium to induce the diffrentiationof apical meristems into plantlets.Theobtained primary plantlets were subjected to virus detection using serologicaland molecular biological methods.Afterconfirming theabsenceofviruses，the plantlets were transferredtopropagationandstrengthening media for multiplication.3 major issues encounteredduringthetissue culture processwere contamination，vitrification，and browning.Contamination，caused by bacteria，fungi，or endophytes，wasprevented through strict aseptic techniques， environmentalsterilization，andtheaditionofantibioticsorbacteriostaticagents totheculturemedium.Virifiedplantlets， characterizedbyatranslucentand fragileappearance，wereprimarilyinducedbyunsuitablecultureconditionsorexcessive subculturing，andcouldbe mitigated byadjusting lightand temperature conditions，regulating hormone concentrations，and incorporating dark culture periods.Browning，a phenomenon where wounded explants secreted brown substances leading to death，was commonly suppressd by adding anti-browning agents such as sodium thiosulfate.This article provides a reference for the production of virus-free seed potatoes
Keywordspotato; virus-free tissue culture;virus detection; biotechnology
马铃薯是茄科茄属块茎类草本植物，是一种粮菜饲兼用作物，具有抗旱、耐寒、耐贫瘠、适应广泛、营养全面、加工用途多和产业链长等优势，对于保障粮食安全具有重要意义。（剩余5797字）
