热水+绿液预处理对竹纤维酶水解制备可发酵糖的影响探究

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关键词：预处理；绿液；纤维素酶；化学助剂；可发酵糖中图分类号：TS74 文献标识码：A DOI： 10.11980/j.issn.0254-508X.2025.11.008

StudyontheEffect ofHotWater + GreenLiguor Pretreatment on the Enzymatic Hydrolysisof Bamboo FiberstoPrepareFermentableSugars

WANG Jian12WANG Xuchong1.2*LI Yong1，²SONG Huan1²YANG Qing1，²DING Ping1.2 ZHANG Ming1，²XU Yaomengli1.2 （1.ZhuzhouTimesNewMaterialTechnologyCo.，Ld.，Zhuzhou，Hu’nanProince，42Oo;2.ZhuzhouTimesFiberPionrMateal Technology Co.，Ltd.，Zhuzhou，Hu'nan Province，412100） （*E-mail：2097986529@qq.com）

Abstract：In this study，powdered single bamboo was pretreated with hot water and hot water + green liquor，combined with chemical additivessuchasbovineserumalbumin（BSA）andTween80.Thebamboofibers wereenzmatichydrolyzedandsaccarfiedbyclulase，and theeectsofpretreamntonditiosndceicaladditisotefecyofzatichdolserevestigatedeultsod that the two-step hot water + green liquor pretreatment had a significant efect on improving the enzymatic hydrolysis efficiency ofbamboo fibers.When the total alkali content of green liquor was 14% ，the total sugar conversion rate after enzymatic hydrolysis of hot water + green liquor pretreated bamboo fibers reached 85.51% . The addition of BSA and Tween 8O significantly increased the yield of fermentable sugars camefrombambofiers，ndtesynegisticfctofthodditioscouldaisethtotalsugarconversioateafterenzaticholyis to 99.67% .Under the combined effects of hot water + green liquor pretreatment and chemical additives，the yield of fermentable sugars per （204号 100g of bamboo raw material could reach 65.88g

Keywords：pretreatment；green liquor;cellulase;chemical additives； fermentable sugars

全球化石能源的过度开发利用导致环境变化和能源短缺，寻找可替代化石燃料的清洁能源刻不容缓3]。（剩余15333字）