2022一2024年中国部分地区牛病毒性腹泻病毒分子检测及基因分型

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中图分类号：S852.653 文献标志码：A 文章编号：0366-6964（2025）10-5328-01

Abstract：Bovine viral diarrhea virus （BVDV） is one of the major pathogens responsible for significant economic losses in catle herds，particularly affecting the health and development of calves. This study aimed to establish a universal SYBR Green I-based real-time quantitative PCR （qPCR） assay for BVDV detection and to investigate the epidemiology， genotypes，and subtypes of BVDV in samples collected from free-range catle herds in selected regions of China. A universal BVDV SYBR Green I qPCR assay was developed based on the 5′ untranslated region （5′UTR） sequences of various BVDV genotypes obtained from GenBank. Using this method，a total of 712 serum samples from free-range catte，as well as tissue samples from calves exhibiting symptoms such as coughing，fever，and diarrhea collected between June 2O22 and December 2O24，were tested and subjected to phylogenetic analysis. Additionally，virus isolation，indirect immunofluorescence assay （IFA），and hematoxylin-eosin （HE） staining were performed on calf samples. The established BVDV SYBR Green I qPCR assay demonstrated high sensitivity，strong specificity，good linearity，and excellent reproducibility. The positive detection rate in serum samples was 19.10% ，and the BVDV-24GS strain was successfully isolated. Phylogenetic analysis of the （20 5′UTR gene sequences revealed that the BVDV strains belonged to subtypes 1c， 1m ，1o，li，lq， 1b ，and 2. These findings indicate the successful development and application of a universal BVDV SYBR Green I qPCR assay，and the presence of varying degrees of BVDV infection among free-range catle in parts of China. The identification of viral genotypes enriches the molecular epidemiological data of BVDV and provides a scientific basis for effective disease control，vaccine development，and optimization of immunization strategies.

Keywords： bovine viral diarrhea virus；SYBR Green I real-time quantitative PCR；molecular detection；genotype identification ∗ Corresponding author：MA Xiaoxia，E-mail： maxiaoxia956@163.com

牛病毒性腹泻（bovineviraldiarrhea，BVD）是由牛病毒性腹泻病毒（bovineviraldiarrheavirus，BVDV）引起的一种重要的牛类疾病，其不仅影响牛的健康和生产性能，且对全球养牛业造成巨大的经济损失[1-2]。（剩余9565字）