基于拉曼光谱分析猪卵母细胞玻璃化冷冻前后变化

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中图分类号：S828.3 文献标志码：A 文章编号：0366-6964（2025）10-4998-09

Abstract： This study aimed to investigate molecular structural changes in porcine mature oocytes before and after vitrification using Raman spectroscopy. Ovaries were collected from pigs at a slaughterhouse，and cumulus-oocyte complexes were isolated in the laboratory. After in vitromaturation，fresh oocytes and vitrified oocytes were collected，respectively. Laser confocal Raman spectrometer was used to obtain the Raman spectrum information of fresh oocytes and vitrified oocytes. The molecular structure changes of oocytes before and after vitrification were analyzed after quality control of the data. The results indicated that，compared to fresh oocytes，vitrified oocytes exhibited reduced carbohydrate components such as N-acetylgalactosamine within the carbohydrate signal range of 1020-1140cm-1 . In the amide III band signal range （1230-1300cm-1. ）， β -sheet content increased markedly while α -helix structures decreased. Within the lipid signal range （2800-3100cm-1; ，the content of long-chain fatty acids decreased，while the content of branched-chain fatty acids increased. In summary，vitrification alters the secondary protein structure，fatty acid composition and other key components like N-acetylgalactosamine in porcine oocytes. These changes result in multiple cryodamage efects，including protein structural damage，altered lipid composition，and abnormal carbohydrate metabolism.

Keywords：porcine；oocyte；Raman spectrum；vitrification

∗ Corresponding authors： PANG Yunwei， E-mail： pangyunwei@caas. cn; WANG Dong， E-mail： dwangcn2002@ vip. sina.com

猪卵母细胞冷冻保存技术在畜牧生产中具有重要应用价值。（剩余16103字）