利用牛子宫内膜上皮细胞和小鼠子宫内膜炎模型评价胎盘组织源脂质体载药复合物的效果

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中图分类号：S859.53 文献标志码：A 文章编号：0366-6964（2025）10-5239-12

Abstract： This study aimed to evaluate the feasibility of placenta-derived phospholipids as carriers for antibacterial drugs and explore their application in the treatment of endometritis.An in vitroinflammatory model of bovine endometrial epithelial cells and an in vivo model of endometritisin mice were established using Escherichia coli （ E .coli），and the therapeutic effect of gentamicin-placenta lipid complex （L-GEN） was evaluated. Results were as follows： Gentamicin liposomes （L-GEN） were prepared by the film dispersion method; the L-GEN suspension was milky white with a bluish tinge and good fluidity. The particle size of L-GEN was measured by a nanoparticle size and zeta potential analyzer to be 293.7nm±2.4nm ，with a polydispersity index of 0.21±0.02 . No significant change in the particle size of L-GEN was observed within 3O days，indicating the stability of the liposomes. Transcriptome sequencing analysis showed that L-GEN had a smaller impact on bovine endometrial cels compared to the control group. KEGG pathway enrichment analysis revealed that L-GEN mainly enriched in the PPAR signaling pathway，affecting cellular lipid metabolism，reducing cell damage and inflammation，and enhancing antibacterial activity. In vivo experiments found that L-GEN could alleviate the congestion and swelling of the mouse uterus tissue and inflammatory cell infiltration after E .coli perfusion，and the expression levels of inflammatory-related genes IL-2，IL-6，IL- 1β ， TNF-α ，and TLR2 decreased（ P⩽ 0.05），thereby alleviating endometrial damage and inflammatory response in mice. Through the endometritis cel model and the in vivo model of endometritis in mice，it was found that the placenta-derived lipid drug delivery complex can effectively alleviate endometrial inflammatory responses and enhance therapeutic effects，providing a reference for the subsequent use of tissue-derived liposomes to carry antibiotics for the treatment of endometritis.

Keywords： gentamicin lipid complex;Escherichia coli ；endometrial cells；endometritis ∗ Corresponding authors： ZHANG Zhiping， E-mail： zhipingzhang@henau. edu. cn; ZHANG Juntao，E-mail：juntaozhang@henau.edu.cn

牛子宫内膜炎会引起发情周期紊乱、产奶量下降、怀孕时间延迟、受胎率降低或丧失繁殖能力等，是导致养牛业经济损失的最主要疾病。（剩余12435字）