脂多糖诱导奶牛子宫内膜上皮细胞焦亡的分析

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中图分类号：S857.23 文献标志码：A 文章编号：0366-6964（2025）10-5232-07

Abstract： This study aimed to analyze the pyroptosis of endometrial epithelial cells induced by lipopolysaccharide（LPS） in dairy cows. Endometrial epithelial cells of dairy cows were treated with 0.1μmol⋅L-1 LPS. The mRNA transcription levels of related inflammatory factors （IL1β ，Nod-1，TNF- α ，IL-18 and IL-6） were detected by real-time fluorescence quantitative PCR （qPCR）.The protein levelsofrelated inflammatory factors（IL ⋅1β ，IL-6 and TNF- α ）weredetected by enzyme-linked immunosorbent assay （ELISA）. The morphological changes of endometrial epithelial cells of dairy cows were further observed by scanning electron microscopy. The expression levels of pyroptosis-related proteins （NLRP3，IL-18，IL- 1β ，GSDMD，Cleaved caspase-1 and Cleaved GSDMD） were detected by Western blot. The results showed that the mRNA transcription levels of inflammatory factors （IL- 1β ，Nod-1，TNF- α ，IL-18 and IL-6）in endometrial epithelial cells of dairy cows increased after LPS stimulation for 12h . Cell swelling and rupture; the expression levels of inflammatory factors IL- ⋅1β ，IL-6 and TNF- α were increased. The expression of pyroptosis and inflammatory-related proteins （NLRP3，IL-18，IL- 1β ，GSDMD，Cleaved caspase-1 and Cleaved GSDMD）was significantly enhanced（ P<0.05 ）. LPS stimulation can induce pyroptosis in bovine endometrial epithelial cells. Keywords：cell damage；endometrial epithelial cells of dairy cows；pyroptosis ∗ Corresponding author： LU Wengeng，E-mail：lwgl712@163.com

子宫是胚胎发育的重要器官，胎儿发育所需要的营养是通过母体与胚胎之间的相互作用供给的，且子宫具有复杂的免疫过程。（剩余11700字）