玉米赤霉烯酮内酯水解酶的研究及其在畜禽日粮中的应用

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中图分类号：S859.8 文献标志码：A 文章编号：0366-6964（2025）10-4839-12

Abstract： Zearalenone （ZEN） and its derivatives rank among the most hazardous mycotoxins worldwide，posing a significant threat to food safety and public health. Consequently，the development of efficient biodegradation strategies for ZEN has emerged as a critical research priority. Among these，enzymatic degradation approaches have garnered considerable attention due to their specificity and eficacy. ZEN-degrading enzymes primarily include laccases， manganese peroxidases，and lactonases. Compared to the first two enzyme classes，lactonases exhibit distinct advantages for the development of ZEN detoxification agents， including higher catalytic activity，welldefined degradation mechanisms，and non-toxic degradation byproducts. However，natural enzymes often suffer from limited stability under industrial processing conditions，hindering their applicability in large-scale production and practical settings. To address this challenge，enhancing enzyme activity and stability through rational or semi-rational design and optimization has become a key research focus. This review comprehensively examines microbial-derived ZEN lactonases， covering gene mining，biochemical characterization，molecular engineering，and practical applications.By evaluating the advantages and limitations of biological detoxification in industrial and livestock production，this analysis provides novel insights for the development of highly active， robust ZEN-detoxifying enzyme preparations.

Keywords： zearalenone；lactonase； rational design；enzyme activity stability； stability optimization；toxin degradation

∗ Corresponding authors： ZHAO Chen，E-mail： zc@ags. ac. cn; WANG Weiwei，E-mail： www ags.ac.cn

农作物在生长、收获和储存及运输过程中，容易受到真菌污染而产生毒素。（剩余25220字）