东乡绿壳蛋鸡与白来航鸡资源群体开产体重、开产日龄性状遗传解析

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中图分类号：S831.2 文献标志码：A 文章编号：0366-6964（2025）10-4903-11

Abstract： The aims of this study was to dissect the genetic architecture of body weight and age at the first egg. Phenotypic data were collected from an F2 segregation population of Dongxiang blue-shelled chickens and White Leghorn. The 1 534 hens in F2 generation were genotyped using 600K gene microarray. The SNPs data were quality cleaned. Missing data was imputed with BEAGLE software. SNPs associated with traits of onset of lay were obtained with single-step GWAS. Gene annotation and GO enrichment analysis were carried out on the significant SNPs to elucidate the biological roles of candidate genes.The body weight at first egg（BWFE） showed high levels of heritability. The heritability on BWFE ranged from O.62 to O.65. The age at first egg（AFE） showed moderate to high levels of heritability.The heritability on AFE ranged from 0.35 to 0.42. The genetic correlation coefficients between traits of onset of lay ranged from O.24 to 0.37. There were one or more QTLs close to HTR2A or CAB39L on chromosome 1 that was significantly associated with the BWFE，which was supported by the additional 343 SNPs over the threshold of significant level. These QTL explained 11.74% and 9.34% of the phenotypic variance of BWFE，respectively. On chromosome 4， region close to NCAPGwas significantly associated with the BWFE，and reinforced by the additional 89 SNPs. The region explained 56% of the phenotypic variance. On chromosome 9，QTL was identified nearby the GPR149 ，and explained 1.33% of the phenotypic variance. Furthermore，QTL on AFE were identified near the STON2 gene on chromosome 5 and the PPMlL gene on chromosome 9，explaining 1.80% and 1.22% of the phenotypic variance of AFE，respectively. Single-step GWAS was used to detect the genetic structure of traits of onset of lay. Two novel QTL，mapped on 40Mb of chromosome 5 and 22Mb of chromosome 9，were identified with BWFE and AFE. GO enrichment analysis showed that the variations on BWFE were related to gonadal development，and the phenotypic variations on AFE was related to calcium ion transport.

Keywords： body weight at first egg; age at first egg； heritability；GWAS;GO enrichment analysis

∗ Corresponding authors： TONG Haibing，E-mail： tonghb @ 163.com；QU Liang，E-mail 676542516@qq.com

开产性状是蛋鸡、肉种鸡常规选育目标。（剩余18515字）