连翘脂苷A通过调控TLR3-NF- σ⋅κB 信号通路抑制H9N2亚型禽流感病毒复制

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关键词：连翘脂苷A；H9N2禽流感病毒；TLR3-NF-kB信号通路；细胞因子;肺微血管内皮细胞；抗病毒治疗；中药中图分类号：S853.74 文献标志码：A 文章编号：0366-6964（2025）10-5266-11

Abstract：This study aimed to investigate the inhibitory effects of Forsythiaside A（FTA） on the replication of H9N2 avian influenza virus （H9N2 AIV） in pulmonary microvascular endothelial cells （PMVECs） and explore its underlying mechanisms. PMVECs infected with H9N2 AIV were treated with varying concentrations of FTA. Cytotoxicity was assessed using the MTT assay， and viral replication was analyzed via RT-qPCR. Protein expresson levels of TLR3，TRIF， and NF-κB were evaluated using Western blot，while cytokine secretion （ TNF-α ，IL-6）and type Iinterferons （ IFN-α ， IFN-β ） were measured through ELISA. The results showed that FTA significantly inhibited the replication of H9N2 AIV（ P<0.05 ）in a dose-dependent manner. It downregulated the overexpression of TLR3，TRIF，and NF-κB proteins （ ⋅P<0. 05 ），suppressed the secretion of pro-inflammatory cytokines （ P<0.05⋅ ），and enhanced the production of type I interferons （ P<0.05， . FTA exhibits significant antiviral activity against H9N2 AIV by modulating the TLR3-NF- κB signaling pathway and regulating cytokine expression， highlighting its potential as a novel host-targeted antiviral agent.

Keywords： forsythiaside A； H9N2 avian influenza virus;TLR3-NF- κB signaling pathway; cytokines;pulmonary microvascular endothelial cells；antiviral therapy； traditional Chinese medicine ∗ Corresponding author： HU Ge，E-mail：bnhuge@126.com

目前，禽流感的防控主要依赖于疫苗，但是类似于H9N2亚型禽流感病毒（H9N2AIV）这样的RNA病毒，因其高突变率和抗原变异性，给疫苗的研发带来了极大挑战[1-2]。（剩余17778字）