基于组学数据筛选康乐黄鸡16周龄体重候选基因与关键通路

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中图分类号：S831.2 文献标志码：A 文章编号：0366-6964（2025）10-4889-14

Abstract： This study aimed to identify functional genes and key signaling pathways related to body weight at 16 weeks of age in Kangle yellow chickens based on omics data， in order to provide a basis for the selection and breeding of local chicken breeds for market weight traits. To detect SNPs significantly associated with body weight at 16-week in 434 Kangle yellow chickens， genome-wide association study （GWAS） was performed using the " Jingxin No. 1" 55K SNP chip. A total of 13 potential SNPs significantly associated with target traits were detected，located on chromosome 3（81 64l 565 bp），chromosome 4（1 860 041 bp，33 409 710 bp， 33 716 131 bp，33 925 946 bp），chromosome 6（23 563 123 bp，35 324 210 bp），chromosome 11 （15 294 113 bp，15 346 007 bp），chromosome 12（2 503 065 bp，2 504 749 bp） and chromosome 18（1 994 686 bp，2 307 607 bp）. The 825 candidate genes near SNP sites were screened. Gene function annotation analysis showed the most significant enrichment of biological processes was motor activity. The significant enrichment of 17 KEGG pathways were found， including tight junction，glycosaminoglycan degradation and metabolic pathways（ P<0.05 ）.Combinedwith previous transcriptomic sequencing data，and published literature，KCNQ5， P 2RY10，ITM2A， RBPMS，PGAM1，CYP17A1，BNIP3，ATMIN，BCO1，TNNC1，GNAI2，MYH1F， MYH1A，MYH10，and PIK3R5 were preliminarily identified as important candidate genes for body weight traits at 16 weeks of age in Kangle yellow chickens. Neuroactive ligand-receptor interaction， metabolic pathway，adrenergic signaling in cardiomyocytes and tight junction were key pathways. In this study，13 SNPs，15 key candidate genes and 4 key pathways were preliminarily identified related to the body weight at 16-week of Kangle yellow chicken. These results provided theoretical basis and technical support for molecular marker-assisted breeding of growth traits in Kangle yellow chickens.

Keywords： Kangle yellow chicken; SNP; GWAS; body weight traits; candidate gene; gene pathway

Corresponding author： XU Jiguo，E-mail： 3425614@qq.com

康乐黄鸡原产于江西省宜春市万载县康乐镇，有着1500多年的养殖历史，耐粗饲、肉质鲜嫩[1]但由于其生长速度慢、生长性状一致性较差，在相同的饲养条件下，地方鸡种的生长速度低于商业品种，导致其价格偏高[2]。（剩余25312字）