西藏地区藏猪源大肠杆菌耐药性及耐药基因检测与分析

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中图分类号：S852.61 文献标志码：A 文章编号：0366-6964（2025）10-5212-10

Abstract： The aim of this study was to investigate the antibiotic resistance of Escherichia coli isolated from Tibetan pigs in Xizang. A total of 18O fecal samples were collected from Tibetan pigs in Shannan， Changdu，and Linzhi in Xizang. Bacterial strains were isolated and identified using bacterial culture，Gram staining，and 16S rRNA gene amplification. Antibiotic resistance was assessed using the K-B disk diffusion method，while virulence genes and resistance genes carried by the strain were detected through PCR amplification. The results showed that，a total of 42 diarrheagenic E. coli strains were isolated from 18O fecal samples，with the overall isolation rates of Enteroaggregative Escherichia coli （EAEC） and atypical Enteropathogenic Escherichia coli （EPEC）being 25.36% and 5.07% ，respectively. Antibiotic resistance analysis showed that EAEC and atypical EPEC exhibited imipenem resistance rates of 97.14% and 100% ，respectively. The most common resistance phenotypes for EAEC and atypical EPEC were TE+DX+MI+ AM+IPM+SIZ and TE+DX+AM+IPM+SIZ ，respectively. All isolates carried the β -lactamase bla TEM gene，with a detection rate of 100.00% . In summary，diarrheagenic E . coli isolated from Tibetan pigs in Xizang exhibited high prevalence and multidrug resistance，with diverse resistance phenotypes and varying levels of resistance gene detection. EAEC was the predominant strain. This study provides valuable data for the prevention and control of diarrheagenic E . coli in Tibetan pigs in Xizang，contributing to the improvement of public health and the optimization of clinical practice.

Keywords：Tibetan pigs；diarrheagenic Escherichia coli；resistance genes ∗ Corresponding author：SHANG Peng，E-mail：nemoshpmh@126.com

藏猪是世界上少有的高原型猪种[1，是我国宝贵的地方品种资源，也是我国国家级重点保护品种中的高原地方猪种，具有适应高海拔恶劣气候环境、抗病、耐粗饲、脂肪沉积能力强等特点[2]。（剩余11623字）