T7噬菌体培养及纯化工艺研究

打开文本图片集

关键词T7噬菌体;培养工艺；纯化工艺；内毒素中图分类号Q939.48 文献标识码A文章编号 0517-6611（2025）20-0088-04doi：10.3969/j.issn.0517-6611.2025.20.019

AbstractTopreparehighqualityTphagecheaplyesearchoncultivationandpurficationprocessesscaredut.Basedoegrowth kineticsofBL21stbacteria，tenteioculationtimingndultiplicityf fection（MOI）forTphagewasoptiedSubsequly， phagewaspropaatedatsalediitialpufanivodulrlratiooeatiodpeipiatiowitlehegllG）. FurtherrefinementwasachievedthroughTitonX-14extractiontoemoveendotoxis.ResultsrevealedthatBL1ostbacterianteredlogarithmic growthseaproxiately.5ousosolatioOptialTageiocatioccedduringthlatelgaritcseatan MOI of 10-4 ， yielding progeny phage concentrations of 6.47×1010 PFU/mL. Ultrafiltration concentration effectively concentrated T7 phage particles while eliminating approximately 90% of endotoxins.PEG precipitation facilitated recovery of around 90% of phage particles from the concentratehilefintlymovigproteistotdotoisrionX-114vrblemperaureetractio，oductedtucd endotoxin content in the final T7 bacteriophage product to 55UE/mL . These findings show that the efficient amplification of T7 phage can be achievedbyogtlureodiiosndthueulpeparatioofhigritcteogeicesviombaiftrafiltration，PEG precipitation，and Triton X-114 extraction methodologies.

KeywordsT7 phage;Cultivation process;Purification process;Endotoxin

1915和1917年分别由英国的EdewardTwort和法国的Felixd'Herele先后发现噬菌体，随后在细菌性疾病的治疗领域得到应用[1]。（剩余8467字）