玉米跨膜蛋白ZmLAZ1家族的信号肽鉴定与分泌功能分析

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关键词玉米；跨膜蛋白；ZmLAZ1；信号肽；鉴定；分泌功能

中图分类号S513文献标识码A

文章编号 0517-6611（2025）20-0001-06

doi： 10.3969/j.issn.0517-6612025.20.001

AbstractObectieToivstigateteologialfuctionadsignalpeptidecharacteristisofemaieLazaus1（LZ）proteset od]Thetransmembranestructureandsignalpeptidesofninemembersof theZmLAZ1familywereanalyzedusingbioiformaticmethod.Te signalpeptidesecretionfuctioasverfedusingaSUC2vectorincombinationwithteyasttrainYK12hichhasasucrosinetase secretiondefect.Result]AllmembersoftheZLAZ1familyontainahig-probabilitytransmembranehelixattheN-terminus.Aongtem， ZmLAZ1-2，ZmLAZ1-3，ZmLAZ1-4，ZmLAZ1-5，ZmLAZ1-7andZmLAZ1-9were predicted toavesignalpeptides.Yeastsecretonexpits furthercnfidthattheZLZ1-7sigalpeptdepossesessecretonactivityandtheZAZ1-7signalpeptidefusionproteinwerepable ofsecretingeraseablingteakdoofoeitoocdes.oclusioissudrovdereticalsuport elucidatingtheologicalfunctiosofZAZfmilyproteinsandtheirfictoalmechanismsinmaie，ndprovidesaeferecefortestudyf gene functions and breeding applications in maize.

Key WordsMaize;Transmembrane protein;，ZmLAZ1;Signal peptide;Identification;Secretion function

玉米是全球种植面积最广、产量最高的粮食作物之一，我国的玉米生产和消费量均居世界第二位[1-2]。（剩余10322字）