培养条件对北苍术不定根生长·多糖积累和降糖活性的影响

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关键词北苍术；不定根；培养条件；多糖；降糖氵中图分类号S567.23文献标识码A文章编号 0517-6611（2025）17-0173-05doi：10.3969/j. issn. 0517-6611. 2025. 17. 036

开放科学（资源服务）标识码（OSID）：

Efect of Different Culture Conditions on Adventitious Roots Growth，Polysaccharide Accumulation，and Hypoglycemic Activityf Atractylodes chinensis

PANGYu-lin，YAOLiu-yangLIUJia-ningetal（KeyLaboratoryofForestPlantEcology，MinstyofEducation，CollgeofChis try，Chemical EngineeringandResource Utilization，NortheastForestry University，Harbin，Heilongjiang150040）

AbstractOectieestdyexplordteectsofultiationcoditiosotegrothlysachaidulationndlc activity of adventitious roots of Atractylodes chinensis. [Method]The adventitious roots of A . chinensis were used as raw materials. The cultivation period （ 7-70d ，increment 7 d），inoculum size（ 6-13g/L ，increment 1g/L ），medium ionic strength（MS，3/4MS，1/2MS，and 1/4MS），types（sucrose，glucose，maoe，fructose）andcocetratios（0，4，50g/L）ofcarbonsourcessellasty（BA NAA）andconcentratios（1，，3mg/L）ofhoonsreivestigate.Tebomasandpolysahaidecontentofthedventitiousroots underdiferenttreatmentseremeasuredResultultiationconditioshadasigificantimpactontegothdpolyaccadeaculation of adventitious roots. The optimal conditions：cultivation period of 49d ，medium ionic strength of 1/2 MS，sucrose of 40g/L ，IBA of （204号 1mg/L ，and inoculum size of 10g/L . The inhibition rate of polysaccharides from adventitious roots of A .chinensis on α -glucosidase was （204号 81.24% and the IC50 value was 0.68mg/mL .[Conclusion] Under ideal conditions，A. chinensis adventitious roots exhibit maximal biomass and polysaccharide accumulation. The polysaccharides of the adventitious roots of A .chinensis strongly inhibit α -glucosidase， suggesting potential for diabetes treatment.

Key wordsAtractylodes chinensis ;Adventitious root; Culture condition;Polysaccharide;Hypoglycemic activity

北苍术[Atractylodeschinensis（DC.）Koidz.]是药食同源的菊科多年生草本植物[1]，主产于我国东北、内蒙古、河北、山东等地[2]。（剩余10212字）