山新杨PdaDREB2E基因的克隆及表达模式分析

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关键词山新杨；非生物胁迫;PdaDREB2E基因；生物信息学分析；基因表达

中图分类号S792.11文献标识码A

文章编号 0517-6611（2025）17-0083-08

doi：10.3969/j.issn.0517-6611.2025.17. 021

开放科学（资源服务）标识码（OSID）：

Abstract To identify the DREB genes in Populus davidiana ×P . alba var. pyramidalis responding to abiotic stress，a PdaDREB2E gene was cloned from Populus davidiana ×P . alba var. pyramidalis in this study.The amino acid sequence characteristics of this gene were analyzed by bioinformatics.Theresultsshowedthat theproteinsequenceofPdaDREB2Econtaied451aminocids，witharelativemolecularassof 50334.94andateoreticaliselectricoit（p）f6.erewereotransmembraneegiosorsigalpeptides，dicatingthatitsao secretedproteiItotaidsrine（S）reone（T）nd5tyosine（Y）posprylationsites.esodarydeayu tures were mainly composed of random coils （41.68% ）， α -helices （34.37%） ），extended chains （14.19% ），and β -sheets（ 9.76% ）. The genewaslocatedintecleusnddsef-activatioactityPlgenetictreaalysisidcatedthatthaoacidsequencealosely relatedtotheEBprotesiopuuslndopuusmeoseexpressopateoftegeindfrentissusndiot ic stresswasanalyzedbyreal-timefluorescence quantitativePCR（RT-qPCR）Theresultsshowedthattheexpressionlevelof PdaDREB2E wasthehighestinrotsandthelowestinstems.Underabiotictresuchasdrought（PG），salt（NaCl），andabscisicacid（ABA）treatment，PdDRBEsoddftexprsioncracterstiesultspridedatealsforthesdyoftetresssistaefucf the PdaDREB2E gene in Populus davidiana ×P . alba var. pyramidalis.

Key words Populus davidiana ×P .alba var. pyramidalis;Abiotic stress;PdaDREB2E gene; Bioinformatics analysis;Gene expression

植物经常受到多种逆境胁迫，导致其生长发育受到影响，甚至死亡。（剩余15521字）