基于DNA条形码的椴树属植物系统发育研究

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关键词椴树属;DNA条形码技术；系统发育中图分类号Q943.2文献标识码A文章编号 0517-6611（2025）17-0103-010doi：10.3969/j.issn.0517-6611.2025.17. 024开放科学（资源服务）标识码（OSID）：

Phylogenetic Study of Tilia Plant Based on DNA Barcoding

JI Xiao-jing，JHong-taoLUAZhi-uietal（CoegeofLifeiences，hangcunNoalUniversityhangchun，lin01） AbstractTheexperimentadoptedDAbarcodetechnolgytoconductphylgeneticresearchonTiliaplantconstructedapylogetictr ofthegenus Tilia.Theresultsshowedthatthegenesequencelengthobtaindbyprimeramplficationofsample1，Tiliamurensis，was ，with A（adenine）content of 18.96% ，T（thymine）content of 19.41% ，C（cytosine） content of 32.51% ，and G（guanine） content of 29.12% ，with an average A+T content of 38.37% and an average G+C content of 61. 63% ： A+T is much lower than G+C ;the length of the gene sequence obtained by primer amplification for sample 2，Tilia mandshurica，was 770bp ，with A content of 42.99% ，T content of （20 30.26% ，C content of 13.51% ，and G content of 13.25% . The average A+T content was 73.25% ，and the average G+C content was 26.75% ，with A+T being much higher than G+C ;the gene sequence length of sample 3， Tilia miqueliana， obtained by primer amplification was 810bp ，with A content of 21. 48% ，T content of 20.00% ，C content of 29.63% ，and G content of 28.89% . The average A+T content was 41.48% ，and the average G+C content was 58.52% ： A+T was lower than G+C . Gene sequence analysis was conducted using dnastar 7.Osoftware，dtreepygenetictrsereostructedteteinethegeneticelatioshipetweteexperimetalamplesdplants intheTilapiaceae.TeAbarcodetechnolgynotonlyprovidesconclusiveevidenceforclarifyingthepylogeneticelatioshipsbetwen plants，but also provides theoretical support for efcient analytical frameworks for other species.

Key Words Tilia Linn. ;DNA barcode technology;Phylogeny

椴树属（TiliaLinn.），隶属于锦葵科，是一类乔木植物。（剩余7706字）