超高效液相色谱-串联质谱法检测保健品中的二氟拉松丙酸酯

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Determination of Diflorasone Propionate in Health Products byUltra PerformanceLiquid Chromatography Tandem Mass Spectrometry

MA Xin， WU Junfa， PAN Zhiqi， QIN Yuxin （Guangzhou Inspectionand Testing Certification Group Co.，Ltd.，National Quality Inspection and Testing Center for Processed Food （Guangdong）， Guangzhou 511447， China）

Abstract： Objective： To establish an ultra-performance liquid chromatography-tandem mass spectrometry method for the determination of diflurazone-17-propionateand diflulazone-2l-propionate in health products. Method： The samples were extracted by ultrasonication or vortexing of acetonitrile and 70% acetonitrile， eluted and separated by Waters BEH- ⋅C18 column （100mm×2.1mm，3.0μm） ， and then determined by electrospray ionization source.Result： The linear relationship between the two diflularsone propionate esters was good in the range of 2.0～ 100.0μg⋅L-1 ，and the correlation coefficient R2⩾0.9987. ，and the average recoveries were 72.3%～92.3% and the relative standard deviations were 4.43%～8.09% under the three spike levels.The limit of detection of solid tablets was 15μg⋅kg-1 and the limit of quantification was 40μg⋅kg-1 . The limit of detection and quantification of oil-based capsules was 30μg⋅kg-1 and 100μg⋅kg-1 .Conclusion： This method has short time， low solvent consumption， high recovery，sensitivity and precision，and can accurately analyze difulasone propionate in health care products.

Keywords： ultra-performance liquid chromatography-tandem mass spectrometry; health; simultaneous detection; diflulasone propionate

二氟拉松（Diflorasone）及其酯类作为一种高效的皮质类固醇激素制剂，展现出卓越的抗炎、抗瘙痒特性，同时兼备显著的免疫抑制与缩血管功能，因此在医学领域中被广泛应用于治疗多种顽固性与复杂性皮肤病[1-2]，为患者提供了有效的缓解与治疗方案，显著改善了患者的皮肤健康状况。（剩余4034字）