基于STAT3/Survivin通路探讨银屑平丸含药血清对NETs诱导HaCat细胞增殖迁移作用的影响

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[关键词］银屑病；银屑平丸；中性粒细胞胞外诱捕网；STAT3/Survivin信号通路；人角质形成细胞 [中图分类号]R285.5 [文献标志码]A [文章编号]doi：10.3969/j.issn.1674-070X.2025.08.005

Effects of Yinxieping Pill-containing serum on NETs-induced proliferation and migration of HaCaT cells via the STAT3/Survivin pathway

WANG Qi’， XI Jianyuan²， ZHANG Yufeng'， XIE Wenfang²，QI Lin²， YANG Xian²， CHEN Bangdi²， LI Xiaopeng2* 1. Hunan Universityof Chinese Medicine， Changsha， Hunan 4102O8， China; 2.The First Hospital of Hunan University ofChinese Medicine，Changsha， Hunan 41ooo7，China

[Abstract]Objective To explorethe mechanismbywhich Yinxieping Pill（YXPP）-containingserum inhibitsthesignal transducerandactivatoroftranscription3 （STAT3）Survivinsignalingpathwayinneutrophilextracellartraps （NETs）-induced proliferationandmigrationofHaCaTcels.MethodsNETswereisolatedfromtheperipheralvenousbloodof psoriasis（PsO） patientsandhealthycontrols （HC）.Blankserum，YXPP-containingserum，andacitretin-containingserumwerepreparedfollowing the methodofserumpharmacologyinChinese medicine.HaCaTcellswereassigned intothecontrolgroup，HC-NETsgroup，PsO NETs group，blank serum group，YXPP-containing serum group，acitretin-containing serum group，and YXPP-containing serum + （20 colivelinTFAgroup.TheprolfrationandmigrationabilitiesofHaCaTcelsineachgroupwerecheckedbyCCK-8assyand scratch wound healing assay. The protein expression levels of STAT3， p -STAT3， and Survivin were measured by Western blot.

ResultsComparedwitheotrolgoupteproliferatioabilityigratonate，andproteinexpressonevelsofSA，p， and Survivin were significantly increased in the PsO-NETs group and blank serum group （ P <0.01）.Compared with the PsO-NETs group，theYXPP-containingserumgroupshowedsignificantlyreducedproliferationabilty，migrationrate，andproteinexpression levels of STAT3， p-STAT3，and Survivin （P<0.05， P<0.01 ）. Compared with the YXPP containing serum group， the YXPP-containing serum + Colivelin TFA group showed significantly higher cell proliferation ability and migration rate （ P <0.01），and protein expression levels of STAT3，p-STAT3，andSurvivin（PO05，PO01）.ConclusionYXPP-containingserumcaninhibitNETs-ndcedproliferation andmigrationof HaCaTcelsanditsmechanismof actionmayberelatedtothesuppressionof theSTAT3/Survivinsignaling pathway.

[Keywords]psoriasis；YinxiepingPill；neutrophil extracelulartraps；STAT3/Survivinsignalingpathway；humankeratinocyte

银屑病（psoriasis，PsO）是一种免疫介导的慢性炎症性疾病，表现为红色斑块表面覆有银白色鳞屑，严重时可全身分布2。（剩余12527字）