凤梨释迦组培快繁体系的建立

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中图分类号 S667.9 文献标识码A 文章编号 1007-7731（2025）17-0050-04

DOI号10.16377/j.cnki.issn1007-7731.2025.17.014

Establishment of a tissue culture rapid propagation system for Annona × atemoya

WU Ailong YANG Lidong HUANG Yueping PANG Kaiyun LI Yinghua （Zhanjiang Preschool Teachers College，Zhanjiang 524Ooo， China）

Abstract To establish a rapid propagation system for the tissue culture of Annona × atemoya，in this experiment， the young and segmented stem segments of the plant were usedas explants to determine the induction rate and diffrentiationrateofcalus，theproliferation multipleof adventitiousbudsandtherootingrateof media withdiffrent hormone ratios.The results showed that the optimal callus induction medium for tissue culture of Annona × atemoya was MS+6-BA 0.5 mg/L +2 ，4-D2 mg/L + sucrose 3O g/L+agar 6 g/L，with an induction rate of 62.50% . The optimal differentiation medium was MS + 6-BA 2.O mg/L+NAA 0.2 mg/L+sucrose 30 g/L+agar 6 g/L， with a differentiation rate of 65.00% . Theoptimal proliferationmediumis MS+6-BA 1.0 mg/L + NAA 0.2 mg/L + sucrose 30 g/L+agar6 g/L，with a proliferation coefficient of 4.2. The optimal rooting medium is 1/2 MS + NAA 0.2 mg/L+sucrose 20 g/L + agar 6 g/L， with a rooting rate of 83.75% . The survival rate of tissue culture seedlings after transplantation is over 90% . In conclusion，this study established a key technical system for tissue culture propagation of Annona × atemoya， providing a reference for the factory and standardized production of sugar apple seedlings.

Keywords Annona × atemoya; tissue culture;rapid propagation system; callus inductior

凤梨释迦 （Annona×atemoya） 是番荔枝科番荔枝属植物，属热带亚热带多年生半落叶性小乔木植物1。（剩余4801字）