施加鸡粪水热炭液氮肥对旱地土壤碳、氮和磷功能基因的影响

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中图分类号：S831.6文献标识码：A 文章编号：0488-5368（2025）07-0046-06

Effects of Hydrothermal Aqueous Phase from Chicken Manure on Functional Genes Involved in Carbon， Nitrogen， and Phosphorus Cycling in Dryland Soil

LIANG Xiaofeng，ZHOU Fangyan，CAO Zixuan，HUANG Hua， （1.SchoolofPetroleumand Environmental Enginering，Yan’anUniversity，Yan'an，Shaanxi716oo，China； 2.Yan'anKeyLaboratoryofAgricultural SolidWasteResource Utilization，Yan'an，Shaanxi716oo，China）

Abstract： Hydrothermal aqueous phase （HAP） from animal manure has the potential to be used as liquid nitrogen fertilizer，but its efectson soil carbon，nitrogen，and phosphoruscycling bacteria are not yet clear.In this study，urea and HAP form chicken manure were appliedas nitrogen fertilizers to drylandsoil，and the diferences in bacterial elemental cycling genes were compared after 37 days of incubation.The results showed that a total of 53 functional genes were detected on the gene chip，with a detection rate of 74.65% . Compared to urea， HAP significantly increased the 16S rRNA and the hydrolysis genes of starch （apu and sga ）and hemicellulose （abfAand xylA），while reducing the chitin hydrolysis gene（ chiA ）and the nitrification gene （amoA2）. Elemental cycling pathway analysis showed that HAP promoted complete denitrification and phosphorus release， whileinhibiting organic nitrogen mineralization，ammonification，and nitrification.Thisstudy provides theoretical references for the utilization ofHAP from animal manure as fertilizer.

Key Words： Animal manure；Hydrothermal carbonization；Hydrothermal aqueous phase；Fertilizer；Func. tional genes

水热炭化技术是一种新兴固体废弃物资源化利用技术，可以快速、高效的将湿生物质固体废物热化学转化成为固相产物水热炭和液相产物炭液[1]。（剩余8970字）