温岭小洋薯组培快繁技术

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中图分类号：S532 文献标志码：A 文章编号：2096-9902（2025）15-0050-05

DOI：10.20028/j.zhnydk.2025.15.012

Abstract：Asthefourth largestglobalfoodcrop，potatoholdssignificantimportanceinensuring foodsecurityand promoting agriculturalidustrializatioTradioaltuberpropgationfacschallngessuchaslongycles，loencandsuscetiblito pestanddiseasetransmisio.Inontrast，theinvitrorapidpropagationtechnologyenablestherapidmuliplicationandlargscale productionofvirus-freesedlingsthroughtisueculture.TopreservethequalityoftheWenling Xiaoyangshupotatothisstudy utilizedWenlingXiaoyangshuasexperimentalmaterialtoinvesigatesterilizationmethods，dferentiationmediumfoulatiosnd subculture medium formulations.Theresultsdemonstrated that the optimal sterilizationprotocol involved 75% ethanol for 30 s combined with 0.1% HgC 12 for 7 min，achieving the lowest contamination rate of 1.67% for explants.The optimized conditions for stem tip callus induction were MS medium supplemented with 6.5g⋅L-1 agar， 30g∙L-1 sucrose， 0.3 mg·L -1 NAA （Naphthaleneacetic acid），and 1.5 mg ·L -1 6 -BA （6-benzylaminopurine），yielding the highest induction rate of 91.67% .For subculture，the optimal medium was MS+6.5 g·L -1 agar+ 30g 山 -1 sucrose+0.1 mg·L -1 NAA+0.1 mg·L -1 6-BA.

Keywords： potato; tissue culture; rapid propagation; germplasm resource protection; breeding

马铃薯是茄科茄属一年生草本植物，其块茎可供食用，是仅次于小麦、水稻、玉米的第四大重要粮食作物。（剩余7119字）