OsABCI7调控水稻种子萌发及生长发育

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关键词水稻;ABC转运蛋白;CRISPR/Cas9;种子萌发;黄化

中图分类号S503.5文献标识码A

文章编号 0517-6611(2025)23-0080-06

doi:10.3969/j.issn.0517-6611.2025.23. 018

开放科学(资源服务)标识码(OSID):

OsABCI7RegulatesSeedGerminationandDevelopmentinRice

LUODao-ying12,IANGGang1,PANBang-hen(1.KeyLaboratoryofTopicalPlantResourcesandSustainable Use,ishuangbanna TropicalBotacaldeaeisg;sitsedef 101400) AbstractObjectie]ToexplorethebiologicalfunctionofOsABCI7inrice.MethodUsingNipponbareastheresearchmatealandthe CRISPR/Cas9editing technology wasutlized todirect editthe OsABCl7 gene to create mutants.[Result]Inabci7-1and abci7 -2 mutants,the OsABCI7eneadfrmeshiftmutaio;omparedwiththeildtype,thesdgeriationrateofthemutantswasslow;durigtevgetative growthstagtutatsidoeoafsoftplatdeasdoolltet;dgeprduciot stage,teplantheightofthemutantsassignificantlylowertanthatoftewildtype.oncusionOABC7isinvolvedinteglatioof riceseedgerminationand growthdevelopment.However,thespecificregulationmechanismstillneeds tobefurtherexplored.

KeyWordsRice;ABC transporters;CRISPR/Cas9;Seed germination;Chlorosis

ABC转运蛋白是一类含有腺苷三磷酸(ATP)结合盒的转运单元,因此被称为ATP 结合盒蛋白(ATP-bindingcas-sette,ABC),这些蛋白能够利用ATP水解释放的能量驱动物质的主动运输[1]。(剩余12429字)

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