丰富环境对缺血性脑卒中小鼠运动和认知功能影响

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[关键词]环境疗法;卒中;缺氧缺血，脑;动作障碍;认知障碍;囊泡谷氨酸转运蛋白质1;小鼠[中图分类号]R338.2 [文献标志码］A [文章编号] 2096-5532（2025）05-0649-05doi：10.11712/jms.2096-5532.2025.61.154 [开放科学（资源服务）标识码（OSID）][网络出版] https：//link.cnki.net/urlid/37.1517.R.20251105.1058.002；

2025-11-05 16：18：14

Effect of enriched environmentonthe motor and cognitivefunctions of mice with ischemic strokeDONG Shanshan，ZHU Ting， MENG Pingping，WANG Yuyang，WANG Qiang（Departmentof Rehabilitation Medicine，The Afiliated Hospitalof Qingdao University，Qingdao 266003，China）

[Abstract]ObjectiveToinvestigate theefectof enriched environment（EE）onthe motorand cognitivefunctions of mice with middlecerebralarteryocclusion/reperfusion（MCAO/R）and itsunderlying mechanism.MethodsAmouse model of MCAO/R wasconstructed，andthen1daylater，the micewerefedinEEorstandardenvironmentfor14days.The modified Neurological Severity Score（mNSS）and therotarod test wereused to evaluate the motorfunctionof mice；theY-mazetest wa used toevaluatethecognitivefunctionofmice;Westernblotingwasusedtomeasuretheexpresionlevelofvesicularglutamatetransporter1（VGLUT1）in thecortex，striatum，and hippocampusof theipsilateral side.ResultsEE significantlyreduced the mNSS score of MCAO/R mice （ ⋅F=103.900，q=9.551，P<0.001） and increased fall latency in the rotarod test，the percentage of time spent in the novel arm，and the percentage of entries into the novel arm in MCAO/R mice Δ（F=10.110-31.910，q=4.271- （204号 7.769，P<0.05） ，and it also reduced the expresion level of VGLUT1 in thecortex，striatum，and hippocampus of the ipsilateral hemisphere of MCAO/R mice （F=8.141-14.600，q=5.318-7.240，P<0.05） .ConclusionEE can significantly improve the motor and cognitive disorders in mice with MCAO/R，possibly by downregulating the expression of VGLUT1.

[Key words]milieutherapy；stroke；hypoxia-ischemia，brain；movement disorders；cognitiondisorders；vesicularglutamate transport protein1；mice

脑卒中是全球第二大致死原因，也是残疾的主要原因之一[1]。（剩余9053字）