经鼻给枸橡酸铁铵对小鼠嗅球GCase和LIMP-2蛋白表达影响

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[关键词]铁;经鼻给药;嗅球;葡糖苷酰鞘氨醇酶;溶酶体相关膜蛋白质2；小鼠[中图分类号]R338.2 [文献标志码］A [文章编号] 2096-5532（2025）05-0645-04doi：10.11712/jms.2096-5532.2025.61.147 [开放科学（资源服务）标识码（OSID）_[网络出版] https：//link.cnki.net/urlid/37.1517.R.20251103.1403.004; 2025-11-0411：52：55

Effectsfintraasaldmstaoofferricammocitrateothexpressoofgucocebosidaeandlysosoalitegalbae protein-2 intheolfactory bulbs of miceLIANG Meiyu，YUANYu，XIE Junxia，SONG Ning（Departmentof Clinical Laboratory，Shanxi Province Fenyang Hospital，Fenyang O322oo，China）

[Abstract]ObjetiveToinvestigatetheeffctsof intranasal administrationof ferricammonium citrate（FAC）onthe expresion of glucocerebrosidase（GCase）andlysosomalintegral membrane protein 2（LIMP-2）in mouseolfactorybulbs.Methods Forty-eight male C57BL/6 mice were randomly divided into 6 groups with 8 mice in each group. Three groups were given FAC bilateral alternate nasal drops （200mg/kg ）once every other day for1，3，or 6 weeks，respectively.The 3 control groups were given the sameamountofnormalsalineoverthesametimeperiods.Proteinlevelsof lghtchainferrtin，GCase，andLIMP-2inolfactory bulbs weredetected by Western blotting.ResultsCompared withthecontrol groups at1，3，and6 weeks，the expressionof lightchainferitinintheolfactorybulbsofmiceintheexperimental groupsatthecorrespondingtimepointswassigniicantlyincreased Δ：t=6.289-27.270，P<0.001） . There were no significant differences in GCase protein expression between nasal administration of FAC and nasal administration of saline at 1，3，and 6 weeks （ P>0.05; .Compared with nasal administration of normal salinefor1week，theexpressionofLIMP-2inofactorybulbsofmicegivennasaladministrationofFACfor1wkwassignificant lyincreased （t=4.483，P<0.01） ，but there were no significant differences in the expression of LIMP-2 at 3 and 6 weeks （ P> 0.05）．ConclusionTherewassignificantirondepositionintheolfactorybulbsafternasaladministrationofFAC，butitwas insufficient to induce significant dysfunction of GCase or LIMP-2.

[Key words]iron；intranasaladministration；olfactorybulb；glucosylceramidase;lysosomal-asociated membrane protein 2; mice

随着人口老龄化的加剧，帕金森病（PD）发病率在我国增长迅速，我国是全球增速最快的国家[1]。（剩余9463字）