神经肽FF对乳腺浸润性导管癌细胞增殖和袭迁移的影响
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[关键词]乳腺癌;神经肽类;细胞增殖;细胞运动;肿瘤浸润;MAP激酶信号系统[中图分类号]R730.26；R364 ［文献标志码］A [文章编号] 2096-5532（2025）05-0638-07doi：10.11712/jms.2096-5532.2025.61.140 [开放科学（资源服务）标识码（OSID）][网络出版] https：//link.cnki.net/urlid/37.1517.R.20251017.1109.001;
2025-10-17 16：29：29
Effects of neuropeptide FFon proliferation，invasion，and migration of invasive ductal carcinoma cellsCU Yujie，WANG Ting ting，WANGYe，ZHANGChunling，PANG Zongxin（School of Clinical Medicine，Qingdao University，Qingdao 266073， China）
[Abstract]ObjetiveToinvestigate theeffectsof neuropeptideFF（NPFF）ontheproliferation，invasion，and migrationof invasiveductalcarcinoma（IDC）celsand theasociated mechanisms.MethodsWe measured thelevelof NPFF in IDCtissue by enzyme-linkedimmunosorbent assay;determined theexpresionofNPFFandNPFFreceptor2（NPFFR2）inIDCtissuebyimmunohistochemistry；measured NPFF levelsin IDCcelIines（MCF10A，MCF10AT，MCF10DCIS，and MCF10CA1d）by Western blot;assessedtheefects ofNPFFontheproliferation，migration，andinvasionof MCFoAcelsby CellCounting Kit-8assay， scratchassay，andTranswellassay；andmeasuredtheexpressionof proteinsrelated totheextracelllarsignal-regulatedkinase （ERK）1/2 signaling pathwaybyWesternblot.ResultsCompared withthoseinadjacenttissue，theexpresionlevelsof NPFF and NPFFR2 proteins in IDC tissue were significantly increased （ ，both P<0.001 ）. The expression of NPFF protein in IDC tissue was significantly higher than that in ductal carcinoma in situ tissue ⟨t=5.34，P<0.001⟩ . MCF10CA1d cells had significantly higher NPFF and NPFFR2 protein levels than MCF1oA cells （ F=9.92，6.64 ，both P<0.05 ）.Compared with NPFF at 0μmol/L ，NPFF at 20 and 40μmol/L significantly increased cell viability and migration，and NPFFat 80μmol/L significantly increased cell invasion and p-ERK1/2 protein expression Ω（t=4.72，5.84，F=3.56 ，all P<0.05 ）；and cells treated with NPFF at （204 80μmol/L plus BIBP3226 at 80μmol/L exhibited significantly decreased invasion ability compared with the 80μmol/L NPFF group ⋅P<0.05 . NPFFR2 knockout significantly reduced the activation of ERK1/2 induced by NPFF at 80μmol/L （t=6.43，P< 0.05）. ConclusionNPFF is highly expressed in IDC tissue，which promotes the proliferation，migration，and invasion of IDC
cells by activating the ERK1/2 signaling pathway.
[Keywords]breast carcinoma；neuropeptides；cell proliferation；cell movement；neoplasm invasiveness；MAP kinase signaling system
乳腺导管原位癌（DCIS）是局限于乳腺导管系统的肿瘤，是乳腺浸润性导管癌（IDC）的非侵袭性前驱阶段[1]。（剩余12331字）
