维替泊芬对脑缺血损伤早期脑组织和星形胶质细胞的影响

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[关键词] 脑缺血;维替泊芬;星形细胞;YAP信号蛋白质类 [中图分类号] R364.4 [文献标志码] A [文章编号] 2096-532（2025）02-0165-04 doi：10.1712/jms.2096-532.2025.61.04

[Abstract] Objective Toinvestigatetheefectofverteporfin（VP）onbraintisueandastrocytesintheearlystageofcerebralischemicinjury. Methods Atotalof36Sprague-Dawleyratswererandomlydividedintosham-operationgroup （Sham group），midlecerebralarteryoclusionmodelgroup （MCAOgroup），andVPtreatmentgroup （MCAO+VPgroup），with12 ratsineachgroup.TheratsintheMCAOgroupandtheMCAO + VPgroupwereusedtoestablishamodelofMCAO，andafter modeling，theratsintheMCAO + VPgroupweregivenintracerebroventricularinjectionofVP （0.1mg/kg） ），whilethoseinthe Shamgroupweregivenshamoperationalone.The2，3，5-triphenyltetrazoliumchloride（TTC）stainingmethodwasusedtoobserve cerebralinfarctionineachgroup，andimmunofluorescenceasaywasusedtoobservethechangesinglialfibrilaryacidicprotein （GFAP）andYes-asociatedprotein（YAP）inastrocytes. Results TTCstainingshowedthatonday3ofcerebralischemicinjury，theMCAOgroupandtheMCAO+VPgrouphadasignificantlyhigherinfarctvolumethantheShamgroup，andcompared withtheMCAOgroup，theMCAO+VPgrouphadasignificantreductionininfarctvolume ΔF=201.00，P<0.05） ）.Theresultsof immunofluorescenceasayshowedthattheMCAOgrouphadasignificantlyhighernumberofGFAP-positivecelsthantheSham group，andcomparedwiththeMCAOgroup，theMCAO + VPgrouphadasignificantreductioninthenumberofGFAP-positive cels ′F=8.51，P<0.05） ），aswelasasignificantreductioninthenumberofYAP-positivecelsamongtheGFAP-positivecels ⟨F=78.41，P<0.01） ）. Conclusion VPexertsaneuroprotectiveefectintheearlystageofcerebralischemia.

[Keywords] brainischemia;verteporfin;astrocytes;YAP-signalingproteins

脑缺血损伤是临床脑血管病中常见的类型[1]。（剩余7032字）