人脐带间充质干细胞对ARDS小鼠肺损伤作用及机制

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[中图分类号] ; [文献标志码] A 「文章编号]2096-5532（2025）02-0159-06doi：10.1712/jms.2096-532.2025.61.060

[Abstract] Objective Toinvestigatethetherapeuticefectsofhumanumbilicalcordmesenchymalstemcels（HUC-MSCs） ina mouse modelofacuterespiratorydistres syndrome （ARDS）andtheunderlying mechanism. Methods Twenty-four C57BL/6 miceweredividedusingarandomnumbertableintocontrolgroup （Cgroup），ARDSmodelgroup （LPSgroup），and HUC-MSCs+LPSgroup（MSCgroup），witheightmicepergroup.Thelungwet-to-dry（W/D）weightratiowascalculated.The proteinlevelinbronchoalveolarlavagefluid（BALF）wasmeasuredbyusingtheBCA method.BALFcelsortingandcountingwas performedwithSwis Giemsastaining.WithHEstaining，pathologicalchangesinlungtisuewereexamined，andlungtisueinjurywasscored.Thelevelsofinterleukin（IL）- 1β ，IL-6，andtumornecrosisfactor- ⋅α （TNF- α ）inBALFandlungtisueweremeasuredbyenzyme-linkedimmunosorbentasay（ELISA）andquantitativereal-timereversetranscriptionPCR （qRT-PCR）.Thepotentialmechanism wasexploredthroughlungtisueRNAsequencing. Results ComparedwiththeCgroup，theLPSgroup showedathickenedalveolarwal，moreinflammatorycelinfiltrationinthealveolarspace，asignificantlyhigherlunginjuryscore， asignificantlyhigherW/Dratio，asignificantlyhigherBALFproteinlevel，andsignificantlyhigherproteinandmRNAlevelsof IL- 1β ，IL-6，and TNF-α inBALFandlungtisue ⋅F=26.52-192.90，P<0.05） .ComparedwiththeLPSgroup，theMSCgroup showedimprovementsinalveolarwalthickeningandinflammatorycelexudationandsignificantdecreasesinthelunginjuryscore， W/Dratio，BALFproteinlevel，andproteinandmRNAlevelsofIL- 1β ，IL-6，andTNF- ⋅α inBALFandlungtisue ⋅P<0.05 ）. TheRNAsequencingandqRT-PCRresultsindicatedthat MSCsmightimprovelunginjurybyreducingRas-relatedprotein1a （Rap1a），C-X-Cmotifchemokine5 Cxcl5 ），andchemokineligand20 （Ccl20）inchemokinesignalingpathways. Conclusion HUC-MSCsmayameliorateARDSandlunginjuryinmicebydownregulatingRap1a，Cxcl5，and Ccl20 levelsinlungtisue.

[Key words] mesenchymal stem cells； respiratory distress syndrome;lunginjury;rap1 GTP-binding proteins;chemokine CXCL5;chemokineC L20;mice

急 性 肺 损 伤 （ ）/急 性 呼 吸 窘 迫 综 合 征（ ）是由多种肺内外因素引发急性弥漫性肺泡损伤，并导致进行性呼吸困难的难治性和致死性疾病[1]。（剩余8718字）