碳氮比对巨大普里斯特氏菌BZ一95降氮性能影响研究

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关键词：碳氮比；巨大普里斯特氏菌（Priestiamegaterium）；氮累积；同化还原细菌中图分类号：S941 文献标志码：A 文章编号：1004—6755（2025）10-0011-04

Abstract：This study aimed to investigate the denitrification efficiency of the assimilatory giant Pseudomonas BZ-95 under varying carbon一 to一nitrogen ratios to optimize its utilization conditions. Results indicate that BZ-95′ s denitrification capacity and pathways are highly dependent on the carbon一 to一nitrogen ratio. At a C/N ratio of 1：1 ， BZ-95 exhibited slower degradation rates for NO2--N （204 and NO3--N ，with residual NO3--N remaining after 6o hours，though no accumulation of NH4+-N occurred during degradation. At a C/N ratio of 5：1，the degradation rates of NO2--N and NO3--N markedlyaccelerated，achieving 90% degradation efficiency for NO2--N within 2O hours without ammonia nitrogen accumulation. At C/N ratios of 10：1 ， 15：1 and 20：1 ，the degradation rates of NO2--N and NO3--N accelerated further，with NO2--N being completely degraded within 14 hours. At C/N ratios of 15：1 and 20：1 ，nitrate was fully degraded by l4 hours. However，severe ammonium nitrogen accumulation occurred during degradation，with higher carbon一to一nitrogen ratios leading to greater ammonium nitrogen accumulation. In summary， precise regulation of the carbon-to一nitrogen ratio in aquaculture water through carbon source supplementation significantly enhances the denitrification capacity of strain BZ-95 .A C/N ratio of 5：1 represents the optimal condition for BZ-95 to achieve peak denitrification eficiency. This research provides theoretical foundations and practical strategies for employing BZ-95 to control nitrogen pollution in aquaculture water， regulate aquatic environments，and enhance economic benefits in aquaculture.

Key words：Carbon-nitrogen ratio;Priestia megaterium;nitrogen accumulation;assimilatory reduction bacteri

高密度水产养殖易致使水体中氨氮、亚硝酸盐、硝酸盐等含氮化合物过量积累，从而对养殖生物造成毒害[1]。（剩余5132字）