火针通过TRPV1/PKC-δ/p-Akt信号通路调节带状疱疹后神经痛大鼠痛觉敏化的机制研究

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[中图分类号]R245

[文献标志码]A [文章编号]doi：10.3969/j.issn.1674-070X.2025.07.010

Mechanism of fire needling on regulating pain sensitization in rats with postherpetic neuralgia via TRPV1/PKC-S/p-Akt signaling pathway

HE Wei， GUAN Jumei， TANG Xu* Sichuan CollegeofTraditional ChineseMedicine，Mianyang，Sichuan 62looo，China

[Abstract]ObjectiveToexplore thepotential mechanismofactionoffirenedling therapyonratswith postherpetic neuralgia （PHN） basedon the transient receptor potential vanilloid1 （TRPV1/protein kinase C-δ （PKC- δ VphosphorylatedAktprotein Ψ（p -Akt）signalingpathway.MethodsAPHNratmodelwasestablishedbyintraperitonealinjectionofvaniloidreceptoragonist （resiniferatoxin，RTX）.Twentyfoursucessfullymodeledratsereandomlydividedintotemodel group（TX），positiveduggoup （RTX+gabapentin），andfireneedlinggroup（RTX+firenedletherapy），withightratsineachgroup.Anotherighthealthratswere randomlyselectedasthecontrolgroup.Mechanicalpawwithdrawalthreshold（PWTandpawthermalwithdrawallatency（TWL）were employedtoevaluatethedegreeofhyperalgesiainPHNratstreatedwithfireneedlingtherapy.HEstainingwasusedtoassess histopathologicalchangesinthespinaldorsalhornofrats.TUNELstainingwasusedtoexamineapoptosisofGABAergicneuronsin the spinal dorsal horn of rats.ELISA was used to measure the content of tumor necrosis factor- α （TNF- α ）and interleukin- 1β （IL1β）inthespinal dorsalhorntissueofrats.Westernblotwasusedtomeasuretheexpresionsofkeyproteins TRPV1，PKC-and p-Aktithespinal dorsal homtissueofratsineach group.ResultsComparedwiththecontrolgroup，themodel groupexhibited lower PWT values （P<0.O5）， higher TWL values （ P <0.05）， increased quantity of apoptotic GABA cells P<0.05 ），elevatedlevelsofTNF- α （204号 and IL- 1β 1 P <0.05）， and increased expressions of TRPV1， PKC-δ， and p-Akt （ P <0.05）. Compared with the model group，both the positive drug group and the fire needling group showed increased PWT values （ P⋅ 0.05）. Conclusion Fireneedlingtherapyaleviates theinflammatoryresponseofPHNratsbyinhibiting theTRPV1/PKC-8/p-Aktsignaling pathway， thereby relieving pain sensitization in PHN.

[Keywords]postherpeticneuralgia;vaniloidreceptoragonist;firenedling;TRPV1/PKC-8/p-Aktsignalingpathway;tu mor necrosis factor- α ；interleukin- 1β ；pain sensitization

带状疱疹后神经痛（postherpetic neuralgia， PHN）是水痘-带状疱疹病毒再激活导致的特征性临床综合征，以神经支配区域簇集性疱疹伴剧烈神经痛为主要表现，其发病机制涉及复杂的神经病理改变，常导致身体功能障碍、情绪障碍等多系统并发症，严重影响患者的生存质量[I-2]。（剩余16700字）