小麦天冬酰胺合成酶基因家族鉴定及表达

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关键词：小麦（TriticumaestiuumL.）；天冬酰胺合成酶（ASN）；基因家族;生物信息学；鉴定；表达

中图分类号：S512 文献标识码：A

文章编号：0439-8114（2025）11-0195-08

DOI：10.14088/j.cnki.issn0439-8114.2025.11.027 开放科学（资源服务）标识码（OSID）：

Genome-wide identification and expression analysis of the asparagine synthetase gene family in wheat

XUEYu-hang1²，LIU Yi- ⋅ke1，2 ，NINGQiang²，FANG Zheng-wu1（1.Collgeoflure，gtzeUestygou4，Hubea；iuteofFodos，ubcdellSciences，Wuhan 430064，China）

Abstract：Basedonthe proteinsequences oftheasparagine synthetase（ASN）genefamilyfrom Arabidopsis and maize，agenomewideidentificationoftheASNgenefamilyinwheat（TriticumaestiumL.）wasconductedusingbioinformaticsmethods，andacoprehensiveanalysisofthesicochemicalpropertis，onsereddomains，hromosomalocations，ndylgeneticelatisisofits memberswasperfored.Meanhile，theexpresionpatersofASNgenesindiferentwheatorgans，atvariousdevelopmentalstages， andunderbioticandabioticstreses weresystematicallyanalyzedusing transeriptomedata.Theresultsshowedthatall14identified wheatASNfamilymemberswerehydrophilicproteinsanddistributedon11chromosomes.Subcelularlocalizationpredictiondicated thatwheatASNproteinswereprimarilylocatedinthecytoplasm，folowedbythecytoskeletonandmitochondria.Phylogeneticanalysis revealed thatthe wheatASgenescouldbedividedintothre subfamilies，andtheconservedmotifs weresimilaroridenticalamong memberswithinthesamesubfamily.Cis-actingelemenanalysisidentifiedeighttypesofregulatoryelementsinthepromoterregions， mainlyncludingabsisicidsposiveess，erellsposives，oughtiducibilitlightsposiveness，nda olismelements，suggesting tattheheatASNfamilygeneswereinvolednotonlyinresponsestovarousstressesbutalsoinmultiple hormoneregulations.AnalysisofabioticstressresponsesshowedthatunderNaCltreatment，theexpresionofallgenesexcept Ta ！ ASN11 was down-regulated.UnderPEG-siulateddroughtstress，theexpreionofTaASN1，aASN7，andTaASN9 wasdowegulated，while thatofTaASN8，TaASN10，andTASN1wasup-regulated.AfterABAtreatment，，theexpresionofTaASN9wadown regulated，whereas theother geneswereup-regulated.The14 wheatASNgenefamilymembers wereevolutionarilyonservedand widely involved in the regulation of biotic and abiotic stress responses.

KeyWords：wheat（TriticumaestiumL.）；asparaginesynthetase（ASN）；genefamily；bioinformatics；identification；expression

小麦（TriticumaestivumL.）是中国北方重要的主食作物，与水稻和玉米并称为中国三大高产粮食作物，在全球范围内广泛种植，小麦产业的健康发展直接关系到中国社会稳定和粮食安全[1。（剩余11802字）