广西姜黄炭疽病病原鉴定及生物学特性分析

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中图分类号： S435.6;S432.4+4 文献标识码：A

文章编号：0439-8114（2025）11-0080-06

DOI：10.14088/j.cnki.issn0439-8114.2025.11.010 开放科学（资源服务）标识码（OSID）：

Identification and biologicalcharacteristics analysis of the pathogencausing anthracnose on Curcuma longa inGuangxi

ZHAN Xin-jie， YANG Dong-yu， JIANG Ni， HUANG Qi， QIU Zhuo-qiu，WEI Shu-gen，BAI Dan-yu， SONGLi-sha，LIYu-qiong，LIANGWen-jing，HUANGYuan

（GuangxiBotancalGardenofdicialPlants/GuagiKeyLboatofgualitFoatioandUtatioofodirbatialCtefor TCMInheritance and Innovation，Nanning 53oo23，China）

Abstract：ToidentifythepathogenicspeciesresponsibleforanthracnoseonCurcumalongainGuangxiandcharacterizeitsbiological properties，thetisueisolationmethodwasadoptedforpathogenisolation.Pathogenswereidentifiedthroughpathogenicityverification，morphological characterization，andmultigenephylogeneticanalysisincorporating ITS，ACT，andGAPDHsequences，while theirbiologicalcharacteristicsweresiultaneouslydetermined.Theresultsshowedthat47fungalsolateserepurifiedfrodiseased Curcumalonga plants.Acording tocolonymorphologyand microscopic morphology，36isolateswiththe highestisolation frequency andconsistentmorphologywereclassfidintothsaegroup，andtherepresenativestrainJH44-8wasselectedforsubsequntstudies.IntegratedmorpholoicalassessmentandultigenepylogeneticnalysisonfiedthattepahogenasColetotricumcurcumae.Studiesonbiologicalcharacteristicsshowedthatthemyceliaofthisstraingrewbestunderthfollowingconditions：tempeatureof （204号 25-28C ，pHof 7-9，dark environment，andon PSA，SDA，and PDA media;theoptimal carbon source was maltose andtheoptimal nitrogen source was peptone；the lethal condition for its mycelia was treatment at 55‰ for 20 minutes.

KeyWords：Curcuma longaL.；anthracnose；pathogenidentification；Coletotrichumcurcumae；biologicalcharacteristics

姜黄（CurcumalongaL.）是姜科姜黄属的多年生草本植物，以其干燥的根茎和块根入药，是药典中姜黄和郁金的基源植物，具有破血行气、通经止痛的功效1。（剩余8338字）