汉逊酵母增香介质制备条件优化对雪茄烟叶的影响

打开文本图片集

关键词：汉逊酵母（Hanseniaspora uvarum）；烟草花蕾;增香介质;雪茄烟叶;响应面优化

中图分类号：TS453 文献标识码：A

文章编号：0439-8114（2025）11-0144-07

DOI：10.14088/j.cnki.issn0439-8114.2025.11.019 开放科学（资源服务）标识码（OSID）：I

Effect of optimization of preparation conditions of Hanseniaspora uvarum aroma-enhancing medium oncigartobacco leaves

LIHao，LIChuan-zong，CHENXuan-xuan，ZHENGBu-fan，YUJun （TobaccoResearchInstituteofHubeiProvince，Wuhan 43oo3O，China）

Abstract：ThepotentialofHanseniasporauwuarumaroma-enhancing mediumpreparedby Hanseniasporauarumandtobacco flower budstoimprovethequalityofsolid-statefermentedcigarleavesunderoptimizedpreparationconditionswasexplored.Aftersinglefactoroptimizationofcarbonsoure（glucose），nitrogensource（isoleucine）concentrationandinoculationamountduringthpreparation ofaroma-enhancing medium，itwasfoundthatthetotalaromareachedthehighestlevelwhenthe glucoseconcentrationwas 10% of the aroma medium system，the isoleucine concentration was 0.8% ，and the inoculation amount was 2.8% of the weight of cigar tobacco leaves.Afteroptimizing theabove threeconditionsbyresponsesurface methodology，thetotalamountofaromacouldreach 1098.70μg/g Atthis time，glucose in the fermentation broth consumed 54.45g/L ，isoleucine decreased by 0.63g/L ，the number of viable bacteria increasedbyO.78×10CFU/mL，andthecontentofacidsandaldehydesinthevolatilearomasubstancesofthefermentationbrothincreased.Thisresearchcould providenovel strategiesandtheoreticalsupportforthedevelopmentofaroma-enhancing mediumforcigars in China.

Keywords：Hanseniasporauuarum；tobaccobuds；aroma-enhancing medium；cigartobaccoleaves；responsesurfaceoptimization

茄衣在雪茄烟的形成中起着至关重要的作用，通常情况下，茄衣在发酵后叶片会变软，弹性增大，从而更贴切茄套与茄芯，不仅如此，色泽、口感与香气也会得到显著的提升，香气会更加浓郁，与茄芯的香气相互融合，从而展现雪茄烟独特的魅力[1-3]。（剩余9070字）