基于网络药理学和分子对接探讨银花泌炎灵片抗急性肾盂肾炎的作用机制

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Exploring the mechanism of action of Yinhua Miyanling Tablets against acute pyelonephritis based on network pharmacology and molecular docking

Zhang Liurong¹，Liu Yuwei¹，Wang Jia’，Liu Linxuan1，Liu Xingyu1 Liu Jinping1，Wang Cuizhu1，Liu Chuangui²， Hao Xiuhua1 （20 1 School of Pharmaceutical Sciences，Jilin University，Changchun13Oo21;²National andLocal United Engieering R&DCenter of Ginseng Innovative Drugs，Changchun130021 ** Corresponding author：Hao Xiuhua，email：haoxh@ jlu.edu.cn

[Abstract]ObjectiveToanalyseandidentifythechemical componentsof Yinhua Miyanling Tablets，andto explorethe mechanism treatingacutepyelonephritis.MethodsToconductanalysisandidentificationofthechemicalcomponentsinYinhuaMiyanlingTabletsbyintegrationofliqudchromatogapmasspectrometry（LC-M），andtoexloretheposiblemechanissofaction bynetworkpharmacologyandmoleculardockingtechnology.ResultsAtotalof10lchemicalcomponentswerenewlyidentifiedin Yinhua MiyanlingTabletsbyLC-MS.Atotalof26coretargetswerescrenedoutbasedonnetworkpharmacology.Basedonnetwork pharmacologicalstrategy，atotalof1Okeytargetssuchasumornecrosisfactor，28keyactivechemicalcomponentssuchasPuerarin andkeypathwayssuchasadvanced glycationendproducts-receptorforadvancedglcationendproducts（AGE-RAGE）weresreened outforthehypolipidemicefectofYinhua MiyanlingTablets.Themoleculardockingresultsshowthatthebindingenergiesbetweenkey components and the key targets were all less than ，and stable hydrogen bonds can be formed. ConclusionYinhua MiyanlingTabletscontainavarietyofstructuraltypesofchemicalcomponentsthatcouldexertananti-acutepyelonephitiseffectthrough “ multi targets-multi pathways”.

[Key words] YinhuaMiyanling Tablets；Acute pyelonephritis；Chemical components；Network pharmacology；Molecular docking

急性肾盂肾炎是指肾盂黏膜及肾实质的急性感染性疾病，若不能得到及时有效的治疗，后期可并发脓毒血症、感染性休克，或发展为慢性肾盂肾炎、慢性肾衰竭，危及患者生命安全[1]。（剩余10864字）