一株携带耐药基因blacTx-M-27 沙门菌P1-CTX噬菌体-质粒的分离及生物学特性分析

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中图分类号：S852.6 文献标志码：A 文章编号：0366-6964（2025）07-3519-09

Abstract： Pl phage-plasmid has garnered much atention as a carrier of antibiotic resistance genes in Enterobacteriaceae. This experiment was conducted to study the Pl phage-plasmid carrying antibiotic resistance genes whether have biological characteristics such as induction and further transduction. The biological characteristics of P1-CTX phage-plasmid carrying the thirdgeneration cephalosporin resistance gene in Salmonella were studied，including phage titer，optimal multiplicity of infection， one-step growth curve， temperature and acid-alkali stability. The results showed that P1-CTX phage-plasmid could induce complete phage with cleavage ability，the titer was 106PFU⋅mL-1 ， and the optimal multiplicity of infection was 0. 01. P1-CTX phage had good activity below 50∘C and could tolerate strong alkali but not strong acid. It can be infected with Salmonella and Escherichia coli. It has no efect on host growth and is very stable. In conclusion，the above results indicated that P1-CTX phage-plasmid had normal phage biological characteristics and could carry drug resistance genes through transduction and spread horizontally with good stability.

Keywords： Salmonella ； Pl phage-plasmid;antibiotic resistance genes；biological characteristics ∗ Corresponding author： XU Chenggang，E-mail： xucg@ zafu. edu. cn

近年来，由于耐药基因在世界各地细菌间爆炸性传播，耐药病原体对临床细菌性疾病的治疗带来了巨大的挑战。（剩余13859字）