脂肪酸结合蛋白4对卡介苗感染巨噬细胞趋化因子分泌的调控作用

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中图分类号：S855.1 文献标志码：A 文章编号：0366-6964（2025）07-3484-11

Abstract： The aim of this study was to explore the regulatory role of fatty acid-binding protein 4 （FABP4） on chemokine secretion in Bacillus Calmete-Guérin （BCG） infected macrophages. Using mouse macrophage RAW264.7 as in vitro model， the expression of FABP4 was knocked down by siRNA，combined with BCG infection，and the expression of chemokines were detected by fluorescence quantitative PCR，ELISA and other methods. Subsequently，the function of FABP4 was inhibited by FABP4 inhibitor BMS-309403 in C57BL/6J mice，combined with BCG infection. The levels of lung chemokine secretion were detected by Western blot and ELISA. The lung tissue injury was observed by HE staining. The bacterial load in lung tissue of mice was detected by coated plate method. The results showed that the mRNA expressions of chemokines CCL2，CCL3，CXCL2，CXCL10 and CXCL11 of RAW264.7 macrophages infected with BCG were up-regulated in a time and dose dependent manner compared with the control group. The expression was significantly increased （ P<0.05 ）when the multiple of infection was 1O MOI at 12h post infection. Compared with the BCG group，the expression levels of CCL3（ P<0.001 ） and CXCL2 （ P<0.01 ） were significantly down-regulated in FABP4 knockdown combined with the BCG group， while the expression levels of CCL2， CXCL1O and CXCL11 were not significantly diferent. Studies in mice showed that BMS-3O9403 reduced the expression of chemokines CCL3（ P<0.001 ）and CXCL2（ P<0.001 ）in lung tissue and serum of mice，and mitigate the lung tissue injury of C57BL/6J mice after BCG infection. Plate coating found that BMS-3094O3 significantly increased the content of pulmonary bacteria in mice infected with BCG （ P<0.001 ）.In conclusion，FABP4 promoted the expression of chemokines in RAW264.7 macrophages and mouse lung tissue infected with BCG and clearance of lung bacteria.

Keywords： Bacillus Calmette-Guérin；macrophage; fatty acid-binding protein 4；chemokine x Corresponding author： DENG Guangcun， E-mail： dgc@ nxu. edu. cn

结核病（tuberculosis，TB）是由结核分枝杆菌（Mycobacteriumtuberculosis，Mtb）感染引起的可空气传播的严重传染性疾病。（剩余14901字）