金黄色葡萄球菌脂蛋白对奶牛骨髓源巨噬细胞炎症介质分泌及前列腺素E2合成分泌的影响

打开文本图片集

中图分类号：S859.7 文献标志码：A 文章编号：0366-6964（2025）07-3474-10

Abstract： Staphylococcus aureus （S. aureus） triggers an inflammatory response in the host upon invading mammalian systems，but the role of its lipoproteins in cytokine release and PGE2 （204号 synthesis remains unclear. This study used the S. aureus SA113 strain，its lipoprotein-deficient mutant，and the complemented strain to infect bovine bone marrow-derived macrophages （bBMMs），aiming to investigate the role of S . aureus lipoproteins in stimulating cytokine release and PGE2 production in immune cells. In this study，the mechanism of action of S. aureus lipoprotein in inducing the secretion and synthesis of immune cell inflammatory mediators and PGE2 by infecting bovine bone marrow-derived macrophages with SA1l3，S. aureus SA113 isogenic mutant lgt：：ermB Δlgt ）deficient in lipoprotein maturation，and its complemented strain . The results showed that compared to the SA113- infected group，the secretion level of PGE2 significantly decreased after infecting bBMMs with the S . aureus △lgt mutant，while the secretion levels of pro-inflammatory cytokines （ TNF-α and IL1β） and anti-inflammatory cytokine （IL-1O） also significantly decreased. Western blot results indicated that S . aureus lipoprotein influenced the activation of the NF ⋅κB /MAPK pathway in bBMMs and the expression of PGE2 synthetic enzymes COX-2 and mPGES-1. In addition，the expression levels of TLR2，TLR4 and NLRP3 genes in bBMMs were significantly reduced after loss of S . aureus lipoprotein. In conclusion，during S . aureus infection，S. aureus lipoprotein plays a significant role in activating macrophage inflammatory signaling pathways， mediating the secretion of inflammatory mediators，and it is closely associated with the synthesis and secretion of PGE2 . This study offers a theoretical foundation for the clinical use of prostaglandin synthetase inhibitors and non-steroidal anti-inflammatory drugs （NSAIDs） to prevent and treat infections from S .aureus.

Keywords： Sta ph ylococcus aureus ; bovine bone marrow-derived macrophages; prostaglandin E2; lipoprotein；cytokines

∗ Corresponding authors： FENG Shuang，E-mail： fairysshuang 163.com；ZHANG Shuangyi E-mail： shuangyisyau@163.com

金黄色葡萄球菌是一种重要的革兰阳性病原体，可导致哺乳动物细菌感染和脓毒症的发生[1-2]。（剩余13917字）