赖氨酸乙酰化修饰在鸡精液冷冻中的保护作用及机制研究进展

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中图分类号：S831.3 文献标志码：A 文章编号：0366-6964（2025）07-3071-09

Abstract：China has a rich diversity of chicken breeds with 14O native breeds，which harbor numerous excellent genetic traits. The conservation，breeding，and utilization of chicken genetic resources are of significant importance for promoting the independent development of national chicken breeding industry. The ultra-low temperature cryopreservation of semen is one of the most practical techniques for the long-term preservation and efficient utilization of livestock and poultry genetic resources. The semen cryopreservation technology for livestock， particularly for cattle，is relatively well-developed，but the technology was limited in chicken due to the long tails and small heads of spermatozoa，which result in poor freeze resistance. The poor fertility and sustained fertilization ability of chicken frozen sperm limited the application of semen cryopreservation technique in conservation and breeding. Post-translational modification of proteins was an important regulatory pathway through which cells respond to internal and external environmental stimuli. In mature sperm， both transcription and translation activities were largely halted，lysine acetylation modification of proteins might play crucial roles during the cryopreservation of chicken semen. These modifications could be essential for maintaining sperm structural integrity，mitochondrial function stability，and DNA integrity. However，the specific mechanisms underlying these effects remain unclear. This review aimed to provide a reference for investigating the role of lysine acetylation in the cryopreservation of chicken semen and establishing novel cryopreservation methods，and to offer theoretical insights and technical support for the efficient conservation of poultry genetic resources.

Keywords： chicken; semen cryopreservation；sperm； lysineacetylationmodification; germplasm resource

精液超低温冷冻保存技术是长期保存雄性动物繁殖能力的重要手段，在种质资源保存和品种改良等方面具有广阔的应用前景[1]。（剩余17918字）