转录因子CEBPB对猪S冠状病毒体外复制的抑制作用

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中图分类号：S852.659.6 文献标志码：A 文章编号：0366-6964（2025）07-3423-10

Abstract：This study aims to explore the impact of CCAAT/enhancer-binding protein beta （CEBPB） on porcine deltacoronavirus （PDCoV） replication. PDCoV was inoculated in LLC-PK1 cells，and the expresson levels of CEBPB at different time points were detected. Overexpression and knockdown of CEBPB were performed，and their effects on PDCoV replication were assessed using qPCR and Western blot. Furthermore，the role of CEBPB in the PDCoV replication cycle was clarified. Viral proteins that influenced the upregulation of CEBPB were screened using qPCR，and the colocalization of PDCoV viral proteins with CEBPB was observed using confocal microscopy. The results showed that CEBPB expresson increased with prolonged virus infection time. Overexpression of CEBPB significantly inhibited PDCoV replication，while knockdown of CEBPB promoted virus replication. CEBPB mainly acted during the replication stage of PDCoV， and PDCoV Nsp4 could promote CEBPB expression. Confocal microscopy revealed colocalization of Nsp4 and CEBPB. In conclusion，CEBPB could significantly inhibit PDCoV replication in vitro，which provided important data for the development of antiviral drugs based on CEBPB.

Keywords：CEBPB;PDCoV；Nsp4；anti-virus

∗ Corresponding authors： LI Jizong，E-mail： lijizong22 sina.com;LI Bin，E-mail：libinana@126 com;YUAN Xiaomin，E-mail： $＼operatorname { y x m 1 2 3 0 } ＼textcircled { ＼omega }$ hunau. edu. cn

猪δ冠状病毒（porcine deltacoronavirus，PDCoV）是一种新发的猪肠致病性冠状病毒，可感染所有年龄的猪，导致哺乳仔猪严重的水样腹泻、呕吐和死亡[1]。（剩余18735字）