IBDV单克隆抗体的制备及双抗体夹心ELISA检测方法的建立

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中图分类号：S852.65 文献标志码：A 文章编号：0366-6964（2025）07-3433-09

Abstract：The aim of this study was to establish a rapid and effective method for detecting infections bursal disease virus （IBDV），monoclonal antibodies were prepared using the whole IBDV as the immunogen. Double antibody sandwich ELISA method was established using monoclonal antibody 6G3 as the capture antibody and monoclonal antibody 4C12 as the detection antibody for the detection of IBDV. The results showed that the ELISA method had good specificity and no cross-reaction with REV，AEV，ALV or EDSV. The sensitivity test showed that the minimum detectable amount of IBDV is 1.585×103ELD50 .The results of inter and intra plate repeatability tests showed that this method had good repeatability. Forty-nine clinical samples were detected by the double antibody sandwich ELISA method and commercial IBDV antigen detection card，and the coincidence rate was 94.18% . In conclusion，the double-antibody sandwich ELISA method established in this study has good specificity， sensitivity and repeatability，and can be used for rapid detection of IBDV. Thus，laysing the foundation for the development of a commercial IBDV detection kit.

Keywords： IBDV;monoclonal antibody； detection method; double antibody sandwich ELISA ∗ Corresponding authors：TANG Lijie，E-mail： tanglijie $＼textcircled { ＼omega }$ 163.com；HAN Jianchun，E-mail： hanjianchun@hotmail.com

传染性法氏囊病（IBD）是由传染性法氏囊病病毒（IBDV）引起的一种急性、高度接触性传染病[]。（剩余13344字）