利用新型含烟叶培养基鉴定津巴布韦烟草叶面优势微生物群落

关键词烟草培养基；津巴布韦烟叶；宏基因组；优势微生物群落

中图分类号 文献标识码A

文章编号 0517-6611（2025）08-0001-06

doi：10.3969/j.issn.0517-6611.2025.08.001

开放科学（资源服务）标识码（OSID）：

AbstractIndetetrentifytedominantmrorganssonthelaesofZmbabweantbacctobcoompoentsaoctatios （volume fractions） of 0 % 2 . 5 % 5 . 0 % and 1 0 . 0 % were added to simulate the microbial growth environment on the surface of tobacco leaves ， toscreentheoncetratooftbaccoeavessitableficoialgowthtoexploesiableobacocuemedmpreparatioodiiost analyzetheesipooffbctcatistatedf cificmicrorgansms.Thesultssowedthatorespeificmicrobalcommunitiescouldbeisolatedenthetbaccoleafculturemedimwas adjusted to at concentrations of 2 . 5 % and 5 . 0 % after sterilization.Metagenomic sequencing technology was used to determine the communitydistributionofmicrorgansmsontesufaceof tobaccoleavesin Zimbabwe.ItwasfoundtatBacilusadaighrelativeabundancin the culture medium at concentrations of 0 % ， 2 . 5 % and 5 . 0 % ，and after the addition of tobacco to the culture medium，Terribacillus became the dominant species.The genera with higher relative abundance on tobacco culture medium at concentrations of 2 . 5 % and 5 . 0 % included Piscibaillu，Amplsiohd，sofateof were identified，such as Bacillus species of the genus Bacillus，Bacillus veleis and Bacillus subtilis.

Key wordsTobacco-containing medium； Zimbabwean tobacco；Metagenomics；Dominant microbial community

烟草发酵是提升烟草品质的重要过程，未发酵的烟叶具有刺激性气味，而发酵会改善烟叶的香气和口感，利于制成烟草产品[1]。（剩余11278字）