玛卡总皂苷促人视网膜母细胞瘤Y79细胞凋亡作用研究

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中图分类号 R285.5 文献标识码A 文章编号 1007-7731（2025）09-0111-05

DOI号 10.16377/j.cnki.issn1007-7731.2025.09.024

AbstractTo investigate the effct and mechanism of Lepidium meyenii Walp.saponin （LMS） in promoting apoptosis ofhumanretinoblastoma Y79cels，Lepidium meyenii Walp.wasusedas theexperimentalmaterial to extract its saponins.Y79 cells were treated with different concentrations of LMS （O，10O，2OO，400，and 8 0 0 μ g / m L ），and the effcts ofLMSonY79cellproliferationandapoptosisatdiffrent treatmenttimes （24，48，and72h）weredetected.The expression levels of pro-apoptotic factors， including Caspase ， Caspase-8， and Caspase genes and proteins， were measured.The results showed that 2.58 g of total saponins were extracted from 5O g of maca dry powder，with an extraction yield of 5 . 1 6 % . LMS treatment inhibited the proliferation of Y79 cells and induced their apoptosis，with the optimal effect observed at 4 0 0 μ g / m L for 48 h，resulting in a cell viability rate of 5 4 . 0 5 % and an apoptosis rate of 2 1 . 8 7 % .Under these conditions，LMS promoted the expression of Caspase-3，Caspase ，and Caspase-9 genes and upregulated the expression of Cleaved caspase ， Cleaved caspase-8，and Cleaved caspase proteins.In conclusion， LMS can inhibit theproliferation of Y79cellsand induce their apoptosis，and its mechanism maybe related to the upregulation of pro-apoptotic factors Caspase ，Caspase-8， and Caspase

Keywords retinoblastoma; Lepidium meyenii Walp.saponin; cell proliferation; cell apoptosis

视网膜母细胞瘤也称视网膜恶性胶质瘤（Retinoblastoma，RB），是由于抑癌基因RB1突变或缺少引发的婴幼儿眼内恶性肿瘤之一[]。（剩余7300字）