酵母硒缓解黄曲霉毒素B1(AFB1)诱导的鹑肝脏损伤效果研究

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中图分类号 S856 文献标识码A 文章编号 1007-7731（2025）09-0080-07

DOI号10.1637/j.cnki.issn1007-7731.2025.09.018

AbstractTo investigate the potential mitigating effectsof yeast selenium （Se-Y）onaflatoxin B1（AFB1）-induced liver injury inquails，2O-day-oldfemale yelow-feathered quails wererandomlydivided into four groups （1O quails per group）： control group （basic daily ration）， Se-Y group （ 0 . 8 m g / k g Se-Y），AFB1 group （ 3 0 μ g / k g B.W AFB1）， and Se-Y + （204号 AFB1 antagonism group 0 . 8 m g / k g Se B.W AFB1）. Liver histopathological damage，serum biochemical parameters，hepatic oxidative and antioxidant indices，andtheexpresion levelsof genes related tothe Nrf2 signaling pathway were assessed.The results showed that compared with the control group，AFB1 exposure caused the quail livers toexhibitsweling，yellowing，whitening，and fragility.Histopathologicalobservationsrevealed disorderedcell cord arrangements，hepatocyte sweling，and vacuolar degeneration.Serum aspartate aminotransferase （（AST）and alanine aminotransferase （ALT）activities were significantly increased.Inliver tissues，malondialdehyde （MDA）and hydrogen peroxide （ ）levels were markedly elevated， while glutathione peroxidase （GSH-Px）， total superoxide dismutase （TSOD），and catalase （CAT）activities were significantly reduced.Aditionaly，total antioxidantcapacity（T-AOC） was decreased. The expression of Nrf2 signaling pathway related genes （ N r f 2 ，HO-1， NQO-1， SOD1， CAT） was significantly suppressed. Compared with the AFB1 group，the Se-Y + AFB1 antagonism group alleviated AFB1-induced liver histopathological damage，reduced serum AST andALTactivities，and increased hepatic GSH-Px， CAT，and T-SOD activities as well as T-AOC levels.Furthermore，the expression of Nrf2 signaling pathwayrelated genes were upregulated.Inconclusion，AFB1 exposure induces oxidative stress in quail livers and suppresses the expressonof Nrf2 signaling pathway-relatedfactors，leading toliver injury.Se-Ycounteracts AFB1-induced hepatic oxidative damage by enhancing the expresson of Nrf2-related factors，though the precise mechanisms require further investigation.

KeywordsNrf2 signaling pathway; yeast-selenium; aflatoxin B1;quail liver; oxidative stress

鹤鹑在饲养过程中易受到饲料中霉菌毒素的污染，在众多的霉菌毒素中，黄曲霉毒素B1（AFB1）的毒性较大，污染较为严重，AFB1通过采食进入机体后，会导致肝脏、肾脏出现损伤，同时伴随着动物机体抗氧化能力的下降，诱导氧化应激1]。（剩余10516字）