花龄对兔眼蓝莓自花授粉花粉管动力学和胚囊寿命的影响

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关键词蓝莓;花龄；自花授粉;花粉管动力学;胚囊寿命

中图分类号S663.9 文献标识码A 文章编号1007-7731（2025）09-0015-05

DOI号10.16377/j.cnki.issn1007-7731.2025.09.004

AbstractTo investigate the effectofflower ageon the growth ofself-pollinated pollen tubesand the lifespan of embryo sacs in rabbit-eye blueberries，‘Powderblue’rabbit-eye blueberrywas usedas experimental material，the flowersof differentflowerages （O，1，2，3，4，5，6，and7daysaftermaleremoval） wereself-pollinated.Andthethe germination of pollen，growth of pollen tube，and embryosac longevity development were observed.The results showed thatflowerage hadasignificant effecton the dynamicsof pollentubes inself-polliationrabbit-eye blueberies.During the ballon flowering period（Odaysafter male removal），self-polination delayed the germination of pollen tubes，but theproportionof styleswith pollen tube growth tothebase was the highest.The larger theflowerage，the lower the proportion of styles with pollen tubes growing to the base.Theratio of callose in theembryo sac increased with the increase of theageofself-pollnating flowers，among them，theratioof callus formation intheovules was lowerfromOto 1dayafter maleremoval，indicating thattheolder theflowerage，themoreseverethedegenerationofovules in the ‘Powderblue’rabbit-eye blueberry. Comprehensive analysis showed that pollination during the ballon flowering period of‘Powderblue’rabbit-eye blueberry can alleviate self incompatibility to a certain extent.

Keywords blueberry; flower age; self-pollination; pollen tube dynamics; embryo sac longevity

自交不亲和是一种广泛存在于开花植物中的生殖隔离现象，可有效阻止自交，促进杂交，维持基因多样性，保持高度杂合性，是维持植物多样性的重要驱动力。（剩余5120字）