高度近视白内障患者晶状体前囊膜中circRNAs的差异性表达

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Abstract：ObjectiveToanalyzethediferentialexpressionandbiologicalfunctionsofcircRNAsintheanteriorlenscapsulesof highmyopicpatients withcataractandtheirpathogenicrolesinthedevelopmentofthiscondition.MethodsAnteriorlens capsulespecimens werecolected intraoperatively from36patients withage-relatedcataract （ARC）and36highmyopic patients withcataract.Among these，18specimens fromeachgroupwereselected for wholetranscriptomesequencingand biologicalanalysis，and theremaining 36 specimens wereused for validationofcircPDGFRA，circFOXJ3，hsa_circ0004767， hsa_circ_0007528，ciCRIM1，circMAN1A2，circSLC5A3，andcircPTK2expressionsusingRT-qPCR.hsa_circ0007528was selectedforcellexperimentstoexamineitsectsonproliferation，migration，andapoptosisoflnsepithelialcs （s）. ResultsA total of16192 circRNAs were detected inthe specimens from both groups，among which 62 circRNAs were diferentiallyexpressed（29upregulatedand33downregulated）.GOandKEGGanalysesrevealedthatthediferentialy expresedciRAswereprimarilylocalizedinhecoplasm，uleoplasmandedoplasmicticulum，andweeinvoled insignalingpathwaysassociatedwithGapjunctionandtheP3K-Akt，NF-B，Jak-STAT，HF-1andMAPKsigaling pathways.TheceRNAnetwork predictedmultipletarget genes.RT-qPCRvalidationresultswereconsistentwiththe sequencingdata.IntheLECs，upregulationofhsa_cic0o7528significantlyinhibitedcellproliferationandmigratioand obviouslypromotedcellapoptosis.ConclusionTheexpresionprofileofcirRNAsintheanteriorlenscapsuleofhighmyopic patientswithcataract differs from thatofARCpatients.Upregulationof hsa_circ_0o07528 inhibitsLECproliferationand migration and promotes cell apoptosis.

Keywords： highmyopia withcataract;circRNA spectrum; whole transcriptomesequencing; hsa_circ0o7528;lensepithelial cells

高度近视白内障（HMC）是指合并屈光不正以及晶状体混浊的一种眼部疾病，伴有眼轴的过度增长，视力下降，严重影响人们日常生活[1。（剩余17462字）